Complete genome sequence of the potato pathogen Ralstonia solanacearum UY031

被引：23

作者：

Guarischi-Sousa, Rodrigo ^{[1
]}

Puigvert, Marina ^{[2
,3
]}

Coll, Nuria S. ^{[2
,3
]}

Siri, Maria Ines ^{[4
]}

Pianzzola, Maria Julia ^{[4
]}

Valls, Marc ^{[2
,3
]}

Setubal, Joao C. ^{[1
,5
]}

机构：

[1] Univ Sao Paulo, Inst Quim, Sao Paulo, Brazil

[2] Univ Barcelona, Dept Genet, Bellaterra, Catalonia, Spain

[3] CRAG, Bellaterra, Catalonia, Spain

[4] Univ Republica, Fac Quim, Dept Biociencias, Catedra Microbiol, Montevideo, Uruguay

[5] Virginia Tech, Biocomplex Inst, Blacksburg, VA USA

来源：

STANDARDS IN GENOMIC SCIENCES | 2016年 / 11卷

基金：

巴西圣保罗研究基金会;

关键词：

Short genome report; Bacterial wilt; Ralstonia solanacearum; Bacterial plant pathogen; Methylome; Uruguay; SPECIES COMPLEX; DNA METHYLATION; IDENTIFICATION; BACTERIA; STRAINS; BIOVAR-2; DATABASE; PROPOSAL; RACE-3; GENES;

D O I：

10.1186/s40793-016-0131-4

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Ralstonia solanacearum is the causative agent of bacterial wilt of potato. Ralstonia solanacearum strain UY031 belongs to the American phylotype IIB, sequevar 1, also classified as race 3 biovar 2. Here we report the completely sequenced genome of this strain, the first complete genome for phylotype IIB, sequevar 1, and the fourth for the R. solanacearum species complex. In addition to standard genome annotation, we have carried out a curated annotation of type III effector genes, an important pathogenicity-related class of genes for this organism. We identified 60 effector genes, and observed that this effector repertoire is distinct when compared to those from other phylotype IIB strains. Eleven of the effectors appear to be nonfunctional due to disruptive mutations. We also report a methylome analysis of this genome, the first for a R. solanacearum strain. This analysis helped us note the presence of a toxin gene within a region of probable phage origin, raising the hypothesis that this gene may play a role in this strain's virulence.

引用

页数：8

共 50 条

[21] Whole-Genome Sequence and Characterization of Ralstonia solanacearum MLY102 Isolated from Infected Tobacco Stalks [J].

Lin, Guan ;

Gao, Juntao ;

Zou, Junxian ;

Li, Denghui ;

Cui, Yu ;

Liu, Yong ;

Kong, Lingxue ;

Liu, Shiwang .

GENES, 2024, 15 (11)

[22] Potato-Infecting Ralstonia solanacearum Strains in Iran Expand Knowledge on the Global Diversity of Brown Rot Ecotype of the Pathogen [J].

Sedighian, Nasim ;

Taghavi, S. Mohsen ;

Hamzehzarghani, Habiballah ;

van der Wolf, Jan M. ;

Wicker, Emmanuel ;

Osdaghi, Ebrahim .

PHYTOPATHOLOGY, 2020, 110 (10) :1647-1656

[23] Comprehensive genome sequence analysis of Ralstonia solanacearum gd-2, a phylotype I sequevar 15 strain collected from a tobacco bacterial phytopathogen [J].

Xiao, Zhiliang ;

Li, Guangcan ;

Yang, Aiguo ;

Liu, Zhengwen ;

Ren, Min ;

Cheng, Lirui ;

Liu, Dan ;

Jiang, Caihong ;

Wen, Liuying ;

Wu, Shengxin ;

Cheng, Yazhi ;

Yu, Wen ;

Geng, Ruimei .

FRONTIERS IN MICROBIOLOGY, 2024, 15

[24] Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans [J].

Haas, Brian J. ;

Kamoun, Sophien ;

Zody, Michael C. ;

Jiang, Rays H. Y. ;

Handsaker, Robert E. ;

Cano, Liliana M. ;

Grabherr, Manfred ;

Kodira, Chinnappa D. ;

Raffaele, Sylvain ;

Torto-Alalibo, Trudy ;

Bozkurt, Tolga O. ;

Ah-Fong, Audrey M. V. ;

Alvarado, Lucia ;

Anderson, Vicky L. ;

Armstrong, Miles R. ;

Avrova, Anna ;

Baxter, Laura ;

Beynon, Jim ;

Boevink, Petra C. ;

Bollmann, Stephanie R. ;

Bos, Jorunn I. B. ;

Bulone, Vincent ;

Cai, Guohong ;

Cakir, Cahid ;

Carrington, James C. ;

Chawner, Megan ;

Conti, Lucio ;

Costanzo, Stefano ;

Ewan, Richard ;

Fahlgren, Noah ;

Fischbach, Michael A. ;

Fugelstad, Johanna ;

Gilroy, Eleanor M. ;

Gnerre, Sante ;

Green, Pamela J. ;

Grenville-Briggs, Laura J. ;

Griffith, John ;

Gruenwald, Niklaus J. ;

Horn, Karolyn ;

Horner, Neil R. ;

Hu, Chia-Hui ;

Huitema, Edgar ;

Jeong, Dong-Hoon ;

Jones, Alexandra M. E. ;

Jones, Jonathan D. G. ;

Jones, Richard W. ;

Karlsson, Elinor K. ;

Kunjeti, Sridhara G. ;

Lamour, Kurt ;

Liu, Zhenyu .

NATURE, 2009, 461 (7262) :393-398

[25] A unique DNA repair and recombination gene (recN) sequence for identification and intraspecific molecular typing of bacterial wilt pathogen Ralstonia solanacearum and its comparative analysis with ribosomal DNA sequences [J].

Kumar, Aundy ;

Prameela, Thekkan Puthiyaveedu ;

Suseelabhai, Rajamma .

JOURNAL OF BIOSCIENCES, 2013, 38 (02) :267-278

[26] Loop-Mediated Isothermal Amplification of Specific Endoglucanase Gene Sequence for Detection of the Bacterial Wilt Pathogen Ralstonia solanacearum [J].

Lenarcic, Rok ;

Morisset, Dany ;

Pirc, Manca ;

Llop, Pablo ;

Ravnikar, Maja ;

Dreo, Tanja .

PLOS ONE, 2014, 9 (04)

[27] Genome sequencing of four strains of Phylotype I, II and IV of Ralstonia solanacearum that cause potato bacterial wilt in India [J].

Patil, Virupaksh U. ;

Girimalla, Vanishree ;

Sagar, Vinay ;

Chauhan, Rajinder Singh ;

Chakrabarti, Swarup Kumar .

BRAZILIAN JOURNAL OF MICROBIOLOGY, 2017, 48 (02) :193-195

[28] Genome-wide analysis of the potato GRF gene family and their expression profiles in response to hormone and Ralstonia solanacearum infection [J].

Wei, Changhe ;

Yan, Jinli ;

Xu, Pan ;

Wu, Xia ;

Yi, Yan ;

Yue, Xuemei ;

Chen, Caiyan ;

Yan, Lang ;

Yin, Mengmeng .

GENES & GENOMICS, 2024, 46 (12) :1423-1436

[29] Complete Genome Sequence of the Frog Pathogen Mycobacterium ulcerans Ecovar Liflandii [J].

Tobias, Nicholas J. ;

Doig, Kenneth D. ;

Medema, Marnix H. ;

Chen, Honglei ;

Haring, Volker ;

Moore, Robert ;

Seemann, Torsten ;

Stinear, Timothy P. .

JOURNAL OF BACTERIOLOGY, 2013, 195 (03) :556-564

[30] Complete Genome Sequence and Comparative Analysis of the Fish Pathogen Lactococcus garvieae [J].

Morita, Hidetoshi ;

Toh, Hidehiro ;

Oshima, Kenshiro ;

Yoshizaki, Mariko ;

Kawanishi, Michiko ;

Nakaya, Kohei ;

Suzuki, Takehito ;

Miyauchi, Eiji ;

Ishii, Yasuo ;

Tanabe, Soichi ;

Murakami, Masaru ;

Hattori, Masahira .

PLOS ONE, 2011, 6 (08)

← 1 2 3 4 5 →