Universal primers for the PCR-mediated amplification of DNA β -: A molecule associated with some monopartite begomoviruses

被引：403
作者：
Briddon, RW
Bull, SE
Mansoor, S
Amin, I
Markham, PG
机构：
[1] John Innes Ctr Plant Sci Res, Dept Dis & Stress Biol, Norwich NR4 7UH, Norfolk, England
[2] Natl Inst Biotechnol & Genet Engn, Faisalabad, Pakistan
来源：
MOLECULAR BIOTECHNOLOGY | 2002年 / 20卷 / 03期
关键词：
geminivirus; satellite; whitefly; begomovirus; DNA beta;
D O I：
10.1385/MB:20:3:315
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
DNA beta is an approx 1350 nucleotide, single-stranded DNA molecule which has been shown to be associated with some monopartite geminiviruses of the genus Begomovirus. This component requires the helper begomovirus for replication in the cells of host plants and for insect transmission, possibly by transencapsidation. Sequence comparisons of the two available DNA P sequences has identified a highly conserved region upstream of a predicted hairpin structure. Abutting primers designed to this conserved region allows PCR-mediated amplification of the full-length DNA component from total nucleic acid extracts isolated from infected plants originating from a variety of geographically distinct sources and host plants.
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收藏
页码：315 / 318
页数：4
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