Polarization distortion effects in polarimetric two-photon microscopy

被引：38
|
作者：
Schoen, Peter [1 ]
Munhoz, Fabiana [1 ]
Gasecka, Alicja [1 ,2 ]
Brustlein, Sophie [1 ]
Brasselet, Sophie [1 ]
机构：
[1] MOSAIC, Inst FRESNEL, F-13397 Marseille, France
[2] ENS Cachan, Lab Photon Quant & Mol, Inst DAlembert, F-94235 Cachan, France
来源：
OPTICS EXPRESS | 2008年 / 16卷 / 25期
关键词：
D O I：
10.1364/OE.16.020891
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We present a global analysis of experimental factors affecting polarization responses in two-photon inverted microscopy. The role of reflection optics and high numerical aperture focusing is investigated in two-photon fluorescence, which can be extended to other nonlinear processes. We show that both effects strongly distort polarization responses and can lead to misleading extraction of molecular order information from polarimetric measurements. We describe a model accounting for these effects and develop a calibration technique for the determination of polarization parameters in the sample plane using two-photon fluorescence polarimetry in liquids. (C) 2008 Optical Society of America
引用
收藏
页码：20891 / 20901
页数：11
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