Enhancement of the fluorescence intensity of DNA intercalators using nano-imprinted 2-dimensional photonic crystal

被引：22

作者：

Endo, Tatsuro ^{[1
]}

Ueda, China ^{[1
]}

Kajita, Hiroshi ^{[2
]}

Okuda, Norimichi ^{[2
]}

Tanaka, Satoru ^{[2
]}

Hisamoto, Hideaki ^{[1
]}

机构：

[1] Osaka Prefecture Univ, Analyt Chem Grp, Dept Appl Chem, Naka Ku, Sakai, Osaka 5998531, Japan

[2] SCIVAX Corp, NANOBIC, Saiwai Ku, Kawasaki, Kanagawa 2120032, Japan

来源：

MICROCHIMICA ACTA | 2013年 / 180卷 / 9-10期

关键词：

Photonic crystal (PhC); Nano-imprint lithography; DNAs; Fluorescence detection; Intercalators; ENERGY-TRANSFER; SURFACE; SPECTROSCOPY; MICROARRAYS; RAMAN; DYES;

D O I：

10.1007/s00604-013-0997-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We have fabricated polymer-based 2-dimensional photonic crystals that play a key role in enhancing the fluorescence of DNA intercalators. Highly ordered 2-dimensional photonic crystals possessing triangle-shaped and nm-sized hole arrays were fabricated on a 100 mu m thick polymer film using nano-imprint lithography. Samples of double-stranded DNAs (sizes: 4361 and 48502 bp; concentration: 1 pM to 10 nM) were adsorbed on the surface of the 2-dimensional photonic crystal by electrostatic interactions and then treated with intercalators. It is found that the fluorescence intensity of the intercalator is enhanced by a factor of up to 10 compared to the enhancement in the absence of the 2-dimensional photonic crystal. Fluorescence intensity increases with increasing length and concentration of the DNAs. If the 2-dimensional photonic crystal is used as a Bragg reflection mirror, the enhancement of fluorescence intensity can be easily observed using a conventional spectrofluorometer. These results suggest that the printed photonic crystal offers a great potential for highly sensitive intercalator-based fluorescent detection of DNAs.

引用

页码：929 / 934

页数：6

共 24 条

[21] FLUORESCENCE DETECTION IN AUTOMATED DNA-SEQUENCE ANALYSIS [J].