Riboflavin (vitamin B-2) is the precursor of the flavin coenzymes flavin mononucleotide and flavin adenine dinucleotide. In Escherichia coli and other bacteria, sequential deamination and reduction steps in riboflavin biosynthesis are catalyzed by RibD, a bifunctional protein with distinct pyrimidine deaminase and reductase domains. Plants have two diverged RibD homologs, PyrD and PyrR; PyrR proteins have an extra carboxyl-terminal domain (COG3236) of unknown function. Arabidopsis (Arabidopsis thaliana) PyrD (encoded by At4g20960) is known to be a monofunctional pyrimidine deaminase, but no pyrimidine reductase has been identified. Bioinformatic analyses indicated that plant PyrR proteins have a catalytically competent reductase domain but lack essential zinc-binding residues in the deaminase domain, and that the Arabidopsis PyrR gene (At3g47390) is coexpressed with riboflavin synthesis genes. These observations imply that PyrR is a pyrimidine reductase without deaminase activity. Consistent with this inference, Arabidopsis or maize (Zea mays) PyrR (At3g47390 or GRMZM2G090068) restored riboflavin prototrophy to an E. coli ribD deletant strain when coexpressed with the corresponding PyrD protein (At4g20960 or GRMZM2G320099) but not when expressed alone; the COG3236 domain was unnecessary for complementing activity. Furthermore, recombinant maize PyrR mediated NAD(P)H-dependent pyrimidine reduction in vitro. Import assays with pea (Pisum sativum) chloroplasts showed that PyrR and PyrD are taken up and proteolytically processed. Ablation of the maize PyrR gene caused early seed lethality. These data argue that PyrR is the missing plant pyrimidine reductase, that it is plastid localized, and that it is essential. The role of the COG3236 domain remains mysterious; no evidence was obtained for the possibility that it catalyzes the dephosphorylation that follows pyrimidine reduction.
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South China Agr Univ, Coll Life Sci, Ctr Med Plants Res, Guangzhou 510642, Peoples R ChinaSouth China Agr Univ, Coll Life Sci, Ctr Med Plants Res, Guangzhou 510642, Peoples R China
Ye, Peng
Su, Jianmu
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South China Agr Univ, Coll Life Sci, Ctr Med Plants Res, Guangzhou 510642, Peoples R ChinaSouth China Agr Univ, Coll Life Sci, Ctr Med Plants Res, Guangzhou 510642, Peoples R China
Su, Jianmu
Lin, Jianhao
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South China Agr Univ, Coll Life Sci, Ctr Med Plants Res, Guangzhou 510642, Peoples R ChinaSouth China Agr Univ, Coll Life Sci, Ctr Med Plants Res, Guangzhou 510642, Peoples R China
Lin, Jianhao
Li, Yanqun
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South China Agr Univ, Coll Life Sci, Ctr Med Plants Res, Guangzhou 510642, Peoples R China
South China Agr Univ, Coll Forestry, Guangdong Key Lab Innovat Dev & Utilizat Forest Pl, Guangzhou 510642, Peoples R ChinaSouth China Agr Univ, Coll Life Sci, Ctr Med Plants Res, Guangzhou 510642, Peoples R China
Li, Yanqun
Wu, Hong
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South China Agr Univ, Coll Life Sci, Ctr Med Plants Res, Guangzhou 510642, Peoples R China
South China Agr Univ, Coll Forestry, Guangdong Key Lab Innovat Dev & Utilizat Forest Pl, Guangzhou 510642, Peoples R ChinaSouth China Agr Univ, Coll Life Sci, Ctr Med Plants Res, Guangzhou 510642, Peoples R China
机构:
Univ Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
Bihanic, Camille
Bony, Aurelie
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Univ Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
Bony, Aurelie
Gros, Florence
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Univ Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
Gros, Florence
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Conart, Corentin
Fiorucci, Sebastien
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Univ Cote dAzur, Inst Chim Nice UMR 7272, CNRS, F-06108 Nice, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
Fiorucci, Sebastien
Casabianca, Herve
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Univ Lyon, Inst Sci Analyt UMR 5280, CNRS, F-69100 Villeurbanne, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
Casabianca, Herve
Schiets, Frederic
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Univ Lyon, Inst Sci Analyt UMR 5280, CNRS, F-69100 Villeurbanne, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
Schiets, Frederic
Chietera, Giorgiana
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IFF LMR Nat, F-06130 Grasse, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
Chietera, Giorgiana
Boachon, Benoit
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Univ Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
Boachon, Benoit
Blerot, Bernard
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IFF LMR Nat, F-06130 Grasse, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
Blerot, Bernard
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Baudino, Sylvie
Jullien, Frederic
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Univ Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
Jullien, Frederic
Saint-Marcoux, Denis
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Univ Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
机构:
South China Agr Univ, Coll Life Sci, Ctr Med Plants Res, Guangzhou 510642, Peoples R ChinaSouth China Agr Univ, Coll Life Sci, Ctr Med Plants Res, Guangzhou 510642, Peoples R China
Ye, Peng
Su, Jianmu
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South China Agr Univ, Coll Life Sci, Ctr Med Plants Res, Guangzhou 510642, Peoples R ChinaSouth China Agr Univ, Coll Life Sci, Ctr Med Plants Res, Guangzhou 510642, Peoples R China
Su, Jianmu
Lin, Jianhao
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South China Agr Univ, Coll Life Sci, Ctr Med Plants Res, Guangzhou 510642, Peoples R ChinaSouth China Agr Univ, Coll Life Sci, Ctr Med Plants Res, Guangzhou 510642, Peoples R China
Lin, Jianhao
Li, Yanqun
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h-index: 0
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South China Agr Univ, Coll Life Sci, Ctr Med Plants Res, Guangzhou 510642, Peoples R China
South China Agr Univ, Coll Forestry, Guangdong Key Lab Innovat Dev & Utilizat Forest Pl, Guangzhou 510642, Peoples R ChinaSouth China Agr Univ, Coll Life Sci, Ctr Med Plants Res, Guangzhou 510642, Peoples R China
Li, Yanqun
Wu, Hong
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South China Agr Univ, Coll Life Sci, Ctr Med Plants Res, Guangzhou 510642, Peoples R China
South China Agr Univ, Coll Forestry, Guangdong Key Lab Innovat Dev & Utilizat Forest Pl, Guangzhou 510642, Peoples R ChinaSouth China Agr Univ, Coll Life Sci, Ctr Med Plants Res, Guangzhou 510642, Peoples R China
机构:
Univ Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
Bihanic, Camille
Bony, Aurelie
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h-index: 0
机构:
Univ Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
Bony, Aurelie
Gros, Florence
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h-index: 0
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Univ Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
Gros, Florence
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Conart, Corentin
Fiorucci, Sebastien
论文数: 0引用数: 0
h-index: 0
机构:
Univ Cote dAzur, Inst Chim Nice UMR 7272, CNRS, F-06108 Nice, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
Fiorucci, Sebastien
Casabianca, Herve
论文数: 0引用数: 0
h-index: 0
机构:
Univ Lyon, Inst Sci Analyt UMR 5280, CNRS, F-69100 Villeurbanne, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
Casabianca, Herve
Schiets, Frederic
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h-index: 0
机构:
Univ Lyon, Inst Sci Analyt UMR 5280, CNRS, F-69100 Villeurbanne, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
Schiets, Frederic
Chietera, Giorgiana
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h-index: 0
机构:
IFF LMR Nat, F-06130 Grasse, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
Chietera, Giorgiana
Boachon, Benoit
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h-index: 0
机构:
Univ Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
Boachon, Benoit
Blerot, Bernard
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h-index: 0
机构:
IFF LMR Nat, F-06130 Grasse, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
Blerot, Bernard
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Baudino, Sylvie
Jullien, Frederic
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h-index: 0
机构:
Univ Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France
Jullien, Frederic
Saint-Marcoux, Denis
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h-index: 0
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Univ Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, FranceUniv Jean Monnet St Etienne, Lab BVpam UMR 5079, CNRS, F-42023 St Etienne, France