Sensitive determination of specific radioactivity of positron emission tomography radiopharmaceuticals by radio high-performance liquid chromatography with fluorescence detection

被引：8
作者：
Nakao, Ryuji [1 ]
Furutsuka, Kenji [1 ,2 ]
Yamaguchi, Masatoshi [3 ]
Suzuki, Kazutoshi [1 ]
机构：
[1] Natl Inst Radiol Sci, Mol Imaging Ctr, Chiba 2638555, Japan
[2] Sumitomo Accelerator Serv, Tokyo 1418686, Japan
[3] Fukuoka Univ, Fac Pharmaceut Sci, Fukuoka 8140180, Japan
来源：
NUCLEAR MEDICINE AND BIOLOGY | 2008年 / 35卷 / 07期
关键词：
PET radiopharmaceuticals; HPLC; Fluorescence detection; Specific radioactivity; Quality control;
D O I：
10.1016/j.nucmedbio.2008.06.005
中图分类号：
R8 [特种医学]; R445 [影像诊断学];
学科分类号：
1002 ; 100207 ; 1009 ;
摘要：
A sensitive quality control method is often required in positron emission tomography (PET) radiopharmaceutical analysis due to the high specific radioactivity of synthetic products. The applicability of a radio high-performance liquid chromatography (HPLC) method with fluorescence detection was evaluated for a wide variety of PET radiopharmaceuticals. In 29 different radiopharmaceuticals studied, 20 compounds exhibited native fluorescence. These properties enabled sensitive determination of their chemical masses by direct fluorimetric detection after separation by HPLC. For some substances, detection limits were below nanograms per milliliter level, at least 40 times better than current UV absorbance detection. Sufficient reproducibility and linearity were obtained for the analysis of pharmaceutical fluid. Post-column fluorimetric derivatization was also established for the quantitative determination of FDG and CIDG in [F-18]FDG samples. These methods could be applied Successfully to the analysis of PET radiopharmaceuticals with ultra-high specific radioactivity. (C) 2008 Elsevier Inc. All rights reserved.
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页码：733 / 740
页数：8
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