Interpolymer complex microparticles based on polymethacrylic acid-chitosan for oral insulin delivery

被引：41
作者：
Sajeesh, S [1 ]
Sharma, CP [1 ]
机构：
[1] Sree Chitra Tirunal Inst Med Sci & Technol, Biomed Technol Wing, Div Biosurface Technol, Trivandrum 695012, Kerala, India
来源：
JOURNAL OF APPLIED POLYMER SCIENCE | 2006年 / 99卷 / 02期
关键词：
microparticles; template polymerization; insulin; polymethacrylic acid; chitosan;
D O I：
10.1002/app.22311
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
In the present Study, microparticles composed of polymethacrylic acid-chitosan (PMAA-CS) were prepared by a novel interionic gelation method. Free-radical polymerization of rnethacrylic acid was carried out in the presence of CS, using a water-soluable initiator, and application of these microparticles toward oral insulin delivery was evaluated. Microparticles obtained were characterized by scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) Studies. From SEM studies, it was observed that microparticles had an aggregated morphology with size similar to 20 mu m, while FTIR confirmed the presence of ionic interaction between PMAA and CS chains. Protein loading was done by diffusion filling method, and from in vitro release study, it was observed that insulin-loaded microparticles displayed a pH depended release profile at alkaline/acidic pH. Microparticles exhibited Sustained release Of insulin for 3-4 h at neutral pH, and enzyme linked immunosorbent assay (ELISA) proved that encapsulated protein maintained 100% biological activity at neutral pH. Preliminary study suggests that these microparticles can serve as good candidate for oral protein delivery. (c) 2005 Wiley Periodicals, Inc.
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收藏
页码：506 / 512
页数：7
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