ROS accumulation and antiviral defence control by microRNA528 in rice

被引：249

作者：

Wu, Jianguo ^{[1
,2
]}

Yang, Rongxin ^{[3
,4
]}

Yang, Zhirui ^{[1
]}

Yao, Shengze ^{[1
]}

Zhao, Shanshan ^{[1
]}

Wang, Yu ^{[1
]}

Li, Pingchuan ^{[5
]}

Song, Xianwei ^{[3
,4
]}

Jin, Lian ^{[1
]}

Zhou, Tong ^{[6
]}

Lan, Ying ^{[6
]}

Xie, Lianhui ^{[2
]}

Zhou, Xueping ^{[7
]}

Chu, Chengcai ^{[3
,4
]}

Qi, Yijun ^{[8
]}

Cao, Xiaofeng ^{[3
,4
,9
]}

Li, Yi ^{[1
]}

机构：

[1] Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China

[2] Fujian Agr & Forestry Univ, Inst Plant Virol, Fujian Prov Key Lab Plant Virol, State Key Lab Ecol Pest Control Fujian & Taiwan C, Fuzhou 350002, Peoples R China

[3] Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China

[4] Chinese Acad Sci, Natl Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

[5] Agr & Agri Food Canada, Morden, MB R6M 1Y5, Canada

[6] Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Jiangsu, Peoples R China

[7] Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou 310029, Zhejiang, Peoples R China

[8] Tsinghua Univ, Coll Life Sci, Tsinghua Peking Ctr Life Sci, Ctr Plant Biol, Beijing 100084, Peoples R China

[9] Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

来源：

NATURE PLANTS | 2017年 / 3卷 / 01期

关键词：

SMALL RNAS; PLANT DEVELOPMENT; ASCORBATE OXIDASE; STRESS TOLERANCE; ROLES; GENE; PHYTOCHROME; TOBACCO; LIGHT;

D O I：

10.1038/nplants.2016.203

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

MicroRNAs (miRNAs) are key regulators of plant-pathogen interactions. Modulating miRNA function has emerged as a new strategy to produce virus resistance traits(1-5). However, the miRNAs involved in antiviral defence and the underlying mechanisms remain largely elusive. We previously demonstrated that sequestration by Argonaute (AGO) proteins plays an important role in regulating miRNA function in antiviral defence pathways(6). Here we reveal that cleavage-defective AGO18 complexes sequester microRNA528 (miR528) upon viral infection. We show that miR528 negatively regulates viral resistance in rice by cleaving L-ascorbate oxidase (AO) messenger RNA, thereby reducing AO-mediated accumulation of reactive oxygen species. Upon viral infection, miR528 becomes preferentially associated with AGO18, leading to elevated AO activity, higher basal reactive oxygen species accumulation and enhanced antiviral defence. Our findings reveal a mechanism in which antiviral defence is boosted through suppression of an miRNA that negatively regulates viral resistance. This mechanism could be manipulated to engineer virus-resistant crop plants.

引用

页数：7

共 28 条

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