Stabilization of d-amino acid oxidase from Rhodosporidium toruloides by immobilization onto magnetic nanoparticles

被引：24
|
作者：
Hsieh, Hao-Chieh [1 ]
Kuan, I-Ching [1 ]
Lee, Shiow-Ling [1 ]
Tien, Gee-Yeng [2 ]
Wang, Yi-Jen [1 ]
Yu, Chi-Yang [1 ]
机构：
[1] Tatung Univ, Dept Bioengn, Taipei 10452, Taiwan
[2] Tatung Univ, Instrument Ctr, Taipei 10452, Taiwan
来源：
BIOTECHNOLOGY LETTERS | 2009年 / 31卷 / 04期
关键词：
D-Amino acid oxidase; Immobilization; Magnetic nanoparticle; Rhodosporidium toruloides; Stabilization; HYDROGEN-PEROXIDE; ENZYME;
D O I：
10.1007/s10529-008-9894-z
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
d-Amino acid oxidase from Rhodosporidium toruloides was immobilized onto glutaraldehyde-activated magnetic nanoparticles. Approximately four enzyme molecules were attached to one magnetic nanoparticle when the weight ratio of the enzyme to the support was 0.12. After immobilization, the T (m) was increased from 45A degrees C of the free form to 55A degrees C. In the presence of 20 mM H2O2, the immobilized form retained 93% of its activity after 5 h while the free form was completely inactivated after 3.5 h.
引用
收藏
页码：557 / 563
页数：7
相关论文
共 50 条
  • [1] Stabilization of d-amino acid oxidase from Rhodosporidium toruloides by immobilization onto magnetic nanoparticles
    Hao-Chieh Hsieh
    I-Ching Kuan
    Shiow-Ling Lee
    Gee-Yeng Tien
    Yi-Jen Wang
    Chi-Yang Yu
    Biotechnology Letters, 2009, 31 : 557 - 563
  • [2] Stabilization of native and double D-amino acid oxidases from Rhodosporidium toruloides and Trigonopsis variabilis by immobilization on streptavidin-coated magnetic beads
    Wang, Shih-Juei
    Yu, Chi-Yang
    Kuan, I-Ching
    BIOTECHNOLOGY LETTERS, 2008, 30 (11) : 1973 - 1981
  • [3] Stabilization of D-amino acid oxidase from Rhodosporidium toruloides by encapsulation in polyallylamine-mediated biomimetic silica
    Kuan, I-Ching
    Wu, Jian-Cheng
    Lee, Shiow-Ling
    Tsai, Chen-Wei
    Chuang, Chiao-An
    Yu, Chi-Yang
    BIOCHEMICAL ENGINEERING JOURNAL, 2010, 49 (03) : 408 - 413
  • [4] Stabilization of native and double d-amino acid oxidases from Rhodosporidium toruloides and Trigonopsis variabilis by immobilization on streptavidin-coated magnetic beads
    Shih-Juei Wang
    Chi-Yang Yu
    I-Ching Kuan
    Biotechnology Letters, 2008, 30 : 1973 - 1981
  • [5] Purification and characterization of a D-amino acid oxidase active against cephalosporin C from Rhodosporidium toruloides
    Lee, YH
    Chu, WS
    PROCESS BIOCHEMISTRY, 1998, 33 (04) : 461 - 467
  • [6] Oligolamellar vesicles for covalent immobilization and stabilization of D-amino acid oxidase
    Yoshimoto, Makoto
    Yamasaki, Miku
    Okamoto, Masakazu
    Umakoshi, Hiroshi
    Kuboi, Ryoichi
    ENZYME AND MICROBIAL TECHNOLOGY, 2013, 52 (01) : 13 - 19
  • [7] Immobilization-stabilization of the dimeric D-amino acid oxidase from porcine kidney
    Carballares, Diego
    Fernandez-Lafuente, Roberto
    Rocha-Martin, Javier
    PROCESS BIOCHEMISTRY, 2022, 122 : 120 - 128
  • [8] Stabilization of enzymes (D-amino acid oxidase) against hydrogen peroxide via immobilization and post-immobilization techniques
    Fernández-Lafuente, R
    Rodríguez, V
    Mateo, C
    Fernández-Lorente, G
    Arminsen, P
    Sabuquillo, P
    Guisán, JM
    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 1999, 7 (1-4) : 173 - 179
  • [9] D-amino-acid oxidase gene from Rhodotorula gracilis (Rhodosporidium toruloides) ATCC 26217
    Alonso, J
    Barredo, JL
    Diez, B
    Mellado, E
    Salto, F
    García, JL
    Cortés, E
    MICROBIOLOGY-SGM, 1998, 144 : 1095 - 1101
  • [10] Simultaneous synthesis of L-DOPA and oxidation of D-amino acid by specific coupling of a peroxidase to D-amino acid oxidase
    Chen, Yao
    Chu, Hao
    Liu, Wei
    Feng, Wei
    ENZYME AND MICROBIAL TECHNOLOGY, 2019, 121 : 8 - 16
12345