Upgrading Fungal Gene Expression on Demand: Improved Systems for Doxycycline-Dependent Silencing in Aspergillus fumigatus

被引：57

作者：

Helmschrott, Christoph ^{[1
]}

Sasse, Anna ^{[2
]}

Samantaray, Sweta ^{[1
]}

Krappmann, Sven ^{[2
,3
,4
]}

Wagener, Johannes ^{[1
]}

机构：

[1] Univ Munich, Max Von Pettenkofer Inst, Munich, Germany

[2] Univ Wurzburg, Res Ctr Infect Dis, D-97070 Wurzburg, Germany

[3] Univ Klinikum Erlangen, Mikrobiol Inst, Klin Mikrobiol Immunol & Hyg, Erlangen, Germany

[4] Univ Erlangen Nurnberg, D-91054 Erlangen, Germany

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2013年 / 79卷 / 05期

关键词：

RNA INTERFERENCE; IDENTIFICATION; MOLD;

D O I：

10.1128/AEM.03626-12

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Conditional gene expression is key for functional studies in any given microorganism. To allow tight regulation in the pathogenic mold Aspergillus fumigatus, improved versions of the doxycycline-dependent Tet-On system were generated by replacing functional elements of the precursor module, thereby circumventing the former problem of leakiness due to intramolecular recombination.

引用

页码：1751 / 1754

页数：4

共 17 条

[1] The application of Tet repressor in prokaryotic gene regulation and expression [J].

Bertram, Ralph ;

Hillen, Wolfgang .

MICROBIAL BIOTECHNOLOGY, 2008, 1 (01) :2-16

[2] Menacing mold:: The molecular biology of Aspergillus fumigatus [J].

Brakhage, AA ;

Langfelder, K .

ANNUAL REVIEW OF MICROBIOLOGY, 2002, 56 :433-455

[3] Latest developments and in vivo use of the Tet system:: ex vivo and in vivo delivery of tetracycline-regulated genes [J].

Corbel, SY ;

Rossi, FM .

CURRENT OPINION IN BIOTECHNOLOGY, 2002, 13 (05) :448-452

[4] ANALYSIS OF THE REGULATION OF THE ASPERGILLUS-NIDULANS ACUD GENE, ENCODING ISOCITRATE LYASE, BY CONSTRUCTION OF A HYBRID PROMOTER [J].

DELUCAS, JR ;

GREGORY, S ;

TURNER, G .

MOLECULAR & GENERAL GENETICS, 1994, 243 (06) :654-659

[5] Deciphering cell wall integrity signalling in Aspergillus fumigatus: identification and functional characterization of cell wall stress sensors and relevant Rho GTPases [J].

Dichtl, Karl ;

Helmschrott, Christoph ;

Dirr, Franziska ;

Wagener, Johannes .

MOLECULAR MICROBIOLOGY, 2012, 83 (03) :506-519

[6] Testing the efficacy of RNA interference constructs in Aspergillus fumigatus [J].

Henry, Christine ;

Mouyna, Isabelle ;

Latge, Jean-Paul .

CURRENT GENETICS, 2007, 51 (04) :277-284

[7] Essential gene identification and drug target prioritization in Aspergillus fumigatus [J].

Hu, Wenqi ;

Sillaots, Susan ;

Lemieux, Sebastien ;

Davison, John ;

Kauffman, Sarah ;

Breton, Anouk ;

Linteau, Annie ;

Xin, Chunlin ;

Bowman, Joel ;

Becker, Jeff ;

Jiang, Bo ;

Roemer, Terry .

PLOS PATHOGENS, 2007, 3 (03)

[8] Gene targeting in Aspergillus fumigatus by homologous recombination is facilitated in a nonhomologous end-joining-deficient genetic background [J].

Krappmann, S ;

Sasse, C ;

Braus, GH .

EUKARYOTIC CELL, 2006, 5 (01) :212-215

[9] Tools to study molecular mechanisms of Aspergillus pathogenicity [J].

Krappmann, Sven .

TRENDS IN MICROBIOLOGY, 2006, 14 (08) :356-364

[10] Fungal Gene Expression on Demand: an Inducible, Tunable, and Metabolism-Independent Expression System for Aspergillus niger [J].

Meyer, Vera ;

Wanka, Franziska ;

van Gent, Janneke ;

Arentshorst, Mark ;

van den Hondel, Cees A. M. J. J. ;

Ram, Arthur F. J. .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2011, 77 (09) :2975-2983

← 1 2 →