Upgrading Fungal Gene Expression on Demand: Improved Systems for Doxycycline-Dependent Silencing in Aspergillus fumigatus

被引：56

作者：

Helmschrott, Christoph ^{[1
]}

Sasse, Anna ^{[2
]}

Samantaray, Sweta ^{[1
]}

Krappmann, Sven ^{[2
,3
,4
]}

Wagener, Johannes ^{[1
]}

机构：

[1] Univ Munich, Max Von Pettenkofer Inst, Munich, Germany

[2] Univ Wurzburg, Res Ctr Infect Dis, D-97070 Wurzburg, Germany

[3] Univ Klinikum Erlangen, Mikrobiol Inst, Klin Mikrobiol Immunol & Hyg, Erlangen, Germany

[4] Univ Erlangen Nurnberg, D-91054 Erlangen, Germany

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2013年 / 79卷 / 05期

关键词：

RNA INTERFERENCE; IDENTIFICATION; MOLD;

D O I：

10.1128/AEM.03626-12

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Conditional gene expression is key for functional studies in any given microorganism. To allow tight regulation in the pathogenic mold Aspergillus fumigatus, improved versions of the doxycycline-dependent Tet-On system were generated by replacing functional elements of the precursor module, thereby circumventing the former problem of leakiness due to intramolecular recombination.

引用

页码：1751 / 1754

页数：4

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