ESTABLISHMENT OF EIMERIA TENELLA (LOCAL ISOLATE) IN CHICKEN EMBRYOS

被引：8

作者：

Jiang, L. ^{[1
]}

Zhao, Q. ^{[1
]}

Zhu, S. ^{[1
]}

Han, H. ^{[1
]}

Dong, H. ^{[1
]}

Huang, B. ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Minist Agr, Key Lab Anim Parasitol, Shanghai 200241, Peoples R China

来源：

PARASITE | 2012年 / 19卷 / 03期

关键词：

Eimeria tenella; chicken; embryo; in vitro cultivation; vaccine; GAMETOCYTES;

D O I：

10.1051/parasite/2012193285

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Development of an in vitro Eimeria (E.) tenella model could be valuable as a tool for vaccine, coccidiostats or molecular biology research. 1.0 x 10(4) sporozoites per 0.1 mL were inoculated into the allantoic cavity of ten-day-old chicken embryos. The complete life-cycle of E. tenella was accomplished in eight-nine days at 37 degrees C and 70 % humidity. The addition of 100 U insulin to the embryos could remarkably improve the output of oocysts. The development of the parasite within the embryos was systematically observed, allowing guidelines to be set regarding the appropriate times at which different developmental stages of the parasite may be sampled.

引用

页码：285 / 289

页数：5

共 50 条

[1] Isolation and Establishment of Eimeria tenella Populations from Local Broiler Chicken Farms
Loo, Shu-San
Mohamed, Mazlan
Mohd-Taib, Farah Shafawati
Khoo, Choon-Kiat
Wan, Kiew-Lian
SAINS MALAYSIANA, 2022, 51 (06): : 1677 - 1686
[2] EFFECT OF LASALOCID ON THE DEVELOPMENT OF EIMERIA-TENELLA IN CHICKEN EMBRYOS
BALL, SJ
PITTILO, RM
JOHNSON, J
LONG, PL
VETERINARY PARASITOLOGY, 1990, 36 (3-4) : 337 - 341
[3] ATTENUATION OF EIMERIA-TENELLA BY SERIAL PASSAGE IN CHICKEN EMBRYOS
NAKAI, Y
OGIMOTO, K
JAPANESE JOURNAL OF VETERINARY SCIENCE, 1987, 49 (02): : 245 - 249
[4] OPTIMUM CONDITIONS FOR CULTIVATION OF EIMERIA-TENELLA IN CHICKEN EMBRYOS
NAKAI, Y
OGIMOTO, K
KATSUNO, M
JAPANESE JOURNAL OF VETERINARY SCIENCE, 1982, 44 (06): : 951 - 956
[5] Some observations on the adaptation of Eimeria tenella (local isolates) sporozoites on chicken embryos through chorioallantoic membrane
Hafeez, M. Abdul
Akhtar, Masood
Ayaz, M. Mazhar
JOURNAL OF VETERINARY SCIENCE, 2006, 7 (01) : 59 - 61
[6] SOME FACTORS AFFECTING SEVERITY OF INFECTION WITH EIMERIA-TENELLA IN CHICKEN EMBRYOS
LONG, PL
PARASITOLOGY, 1970, 60 : 435 - &
[7] CLONING OF EIMERIA-TENELLA IN THE CHICKEN
CHAPMAN, HD
ROSE, ME
JOURNAL OF PARASITOLOGY, 1986, 72 (04) : 605 - 606
[8] DEVELOPMENT OF EIMERIA TENELLA IN AVIAN EMBRYOS
LONG, PL
NATURE, 1965, 208 (5009) : 509 - &
[9] Plasma biochemical indices at various stages of infection with a field isolate of Eimeria tenella in broiler chicken
Mondal, Dipak Kumar
Chattopadhyay, Saibal
Batabyal, Subhasish
Bera, Asit Kumar
Bhattacharya, Debasis
VETERINARY WORLD, 2011, 4 (09) : 404 - 409
[10] DEVELOPMENT OF EIMERIA-TENELLA IN QUAIL EMBRYOS
ROLLINSON, D
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1976, 70 (01) : 21 - 21

← 1 2 3 4 5 →