Chromatographic separation of fluoxetine hydrochloride enantiomers by cellulose chiral stationary phase

Fluoxetine hydrochloride enantiomers were separated by chromatography on Tris-(3,5-dimethylphenyl carbamate) cellulose as a chiral stationary phase (CSP). The effects of mobile phase composition, flow rate, and column temperature on capacity factor, separation factor, and resolution were studied systematically. Best separation was obtained with a mobile phase composition of hexane/isopropyl alcohol/diethylamine of 98/2/0.2 (v/v/v) at 15 degrees C. The optimal flow rate was 0.24 mL/min from the Van Deemter equation. However, baseline resolution (R S >1.5) was achieved under a flow rate of 0.8 mL/min. Thermodynamic parameters Delta H 0 , Delta S,R Delta H 0 , and Delta S,R Delta S 0 were calculated. In the temperature range examined, the enthalpic contribution to the enantiomer transfer energy was found to be more significant than the entropic one.