Production, extraction and stabilization of lutein from microalga Chlorella sorokiniana MB-1

被引：92

作者：

Chen, Chun-Yen ^{[1
]}

Jesisca ^{[2
]}

Hsieh, Chienyan ^{[3
]}

Lee, Duu-Jong ^{[4
]}

Chang, Chien-Hsiang ^{[2
]}

Chang, Jo-Shu ^{[2
,5
,6
]}

机构：

[1] Natl Cheng Kung Univ, Univ Ctr Biosci & Biotechnol, Tainan 701, Taiwan

[2] Natl Cheng Kung Univ, Dept Chem Engn, Tainan, Taiwan

[3] Natl Kaohsiung Normal Univ, Dept Biotechnol, Kaohsiung, Taiwan

[4] Natl Taiwan Univ, Dept Chem Engn, Taipei 10764, Taiwan

[5] Natl Cheng Kung Univ, Res Ctr Energy Technol, Tainan 701, Taiwan

[6] Natl Cheng Kung Univ, Strategy Ctr, Tainan 701, Taiwan

来源：

BIORESOURCE TECHNOLOGY | 2016年 / 200卷

关键词：

Lutein; Microalgae; Chlorella sorokiniana; Reduced pressure extraction; Stabilization; SUPERCRITICAL-FLUID EXTRACTION; BETA-CAROTENE; SCENEDESMUS; STABILITY;

D O I：

10.1016/j.biortech.2015.10.071

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The efficiencies of extraction and preservation of lutein from microalgae are critical for the success of its commercialization. In this study, lutein was produced by Chlorella sorokiniana MB-1 via semi-batch mixotrophic cultivation. The microalgal biomass with a lutein content of 5.21 mg/g was pretreated by bead-beating and high pressure cell disruption methods, and the lutein content was harvested by a reduced pressure extraction method. The effect of pretreatment, pressure, solvent type, extraction time and temperature on lutein recovery was investigated. Using high pressure pretreatment followed by extraction with tetrahydrofuran (THF) as solvent resulted in high lutein recovery efficiencies of 87.0% (20 min) and 99.5% (40 min) at 850 mbar and 25 degrees C. In contrast, using ethanol as the solvent, 86.2% lutein recovery was achieved under 450 mbar, 35 degrees C and 40 min extraction. The extracted lutein was stabilized in olive oil or sunflower oil with half-lives of 53.1 and 63.8 days, respectively. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：500 / 505

页数：6

共 26 条

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