Mechanisms of coordination of Ca2+ signals in pancreatic islet cells

被引:56
作者
Bertuzzi, F
Davalli, AM
Nano, R
Socci, C
Codazzi, F
Fesce, R
Di Carlo, V
Pozza, G
Grohovaz, F
机构
[1] CNR, Cellular & Mol Pharmacol Ctr, Dept Surg, Unit Metab Dis, I-20133 Milan, Italy
[2] Ist Sci San Raffaele, B Ceccarelli Ctr, Dept Neurosci, Dipartimento Biotecnol, I-20132 Milan, Italy
[3] Ist Sci San Raffaele, Dept Med, I-20132 Milan, Italy
关键词
D O I
10.2337/diabetes.48.10.1971
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Within pancreatic islet cells, rhythmic changes in the cytosolic Ca2+ concentration have been reported to occur in response to stimulatory glucose concentrations and to be synchronous with pulsatile release of insulin. We explored the possible mechanisms responsible for Ca2+ signal propagation within islet cells, with particular regard to gap junction communication, the pathway widely credited with being responsible for coordination of the secretory activity. Using fura-2 imaging, we found that multiple mechanisms control Ca2+ signaling in pancreatic islet cells. Gap junction blockade by 18 alpha-glycyrrhetinic acid greatly restricted the propagation of Ca2+ waves induced by mechanical stimulation of cells but affected neither Ca2+ signals nor insulin secretion elicited by glucose elevation. The source of Ca2+ elevation was also different under the two experimental conditions, the first being sustained by release from inner stores and the second by nifedipine-sensitive Ca2+ influx. Furthermore, glucose-induced Ca2+ waves were able to propagate across cell-free clefts, indicating that diffusible factors can control Ca2+ signal coordination. Our results provide evidence that multiple mechanisms of Ca2+ signaling operate in beta-cells and that gap junctions are not required for intercellular Ca2+ wave propagation or insulin secretion in response to glucose.
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收藏
页码:1971 / 1978
页数:8
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