A glycolytic metabolon in Saccharomyces cerevisiae is stabilized by F-actin

被引:52
作者
Araiza-Olivera, Daniela [1 ]
Chiquete-Felix, Natalia [1 ]
Rosas-Lemus, Monica [1 ]
Sampedro, Jose G. [2 ]
Pena, Antonio [1 ]
Mujica, Adela [3 ]
Uribe-Carvajal, Salvador [1 ]
机构
[1] Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Dept Mol Genet, Mexico City 04510, DF, Mexico
[2] Univ Autonoma San Luis Potosi, Inst Fis, San Luis Potosi, Mexico
[3] Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Dept Cellular Biol, Mexico City, DF, Mexico
关键词
actin; cytoskeleton; enzyme association; glycolytic metabolon; yeast metabolism; BROWNIAN DYNAMICS SIMULATIONS; ENZYME-ENZYME INTERACTIONS; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; STRUCTURAL PROTEINS; YEAST; ALDOLASE; BINDING; ASSOCIATION; TREHALOSE; PHOSPHOFRUCTOKINASE;
D O I
10.1111/febs.12387
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the Saccharomyces cerevisiae glycolytic pathway, 11 enzymes catalyze the stepwise conversion of glucose to two molecules of ethanol plus two CO2 molecules. In the highly crowded cytoplasm, this pathway would be very inefficient if it were dependent on substrate/enzyme diffusion. Therefore, the existence of a multi-enzymatic glycolytic complex has been suggested. This complex probably uses the cytoskeleton to stabilize the interaction of the various enzymes. Here, the role of filamentous actin (F-actin) in stabilization of a putative glycolytic metabolon is reported. Experiments were performed in isolated enzyme/actin mixtures, cytoplasmic extracts and permeabilized yeast cells. Polymerization of actin was promoted using phalloidin or inhibited using cytochalasin D or latrunculin. The polymeric filamentous F-actin, but not the monomeric globular G-actin, stabilized both the interaction of isolated glycolytic pathway enzyme mixtures and the whole fermentation pathway, leading to higher fermentation activity. The associated complexes were resistant against inhibition as a result of viscosity (promoted by the disaccharide trehalose) or inactivation (using specific enzyme antibodies). In S. cerevisiae, a glycolytic metabolon appear to assemble in association with F-actin. In this complex, fermentation activity is enhanced and enzymes are partially protected against inhibition by trehalose or by antibodies.
引用
收藏
页码:3887 / 3905
页数:19
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