DNA delivery via cationic solid lipid nanoparticles (SLNs)

被引：47

作者：

Carrillo, Carolina ^{[1
]}

Sanchez-Hernandez, Noemi ^{[2
]}

Garcia-Montoya, Encarna ^{[1
]}

Perez-Lozano, Pilar ^{[1
]}

Sune-Negre, Josep M. ^{[1
]}

Tico, Josep R. ^{[1
]}

Sune, Carlos ^{[2
]}

Minarro, Montserrat ^{[1
]}

机构：

[1] Univ Barcelona, Fac Pharm, E-08028 Barcelona, Spain

[2] CSIC, Inst Parasitol & Biomed Lopez Neyra, Granada, Spain

来源：

EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES | 2013年 / 49卷 / 02期

关键词：

Non-viral vectors; Lipoplexes; Gene therapy; Solid lipid nanoparticles; Cationic nanoparticles; Drug delivery systems; NONVIRAL GENE-THERAPY; IN-VITRO; TRANSFECTION; BIND; CHITOSAN; VECTORS; ABILITY; DESIGN;

D O I：

10.1016/j.ejps.2013.02.011

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In recent years the use of solid lipid nanoparticles (SLNs) as transport systems for the delivery of drugs and biomolecules has become particularly important. The use of cationic SLNs developed by the technique of microemulsion, which are complexed with DNA in order to study their application as non-viral vectors in gene therapy, is reported. The nanoparticles are characterized by scanning electron microscopy and transmission electron microscopy (SEM and TEM), atomic force microscopy (AFM) and differential scanning calorimetry (DSC). Furthermore, the process of lyophilization of the samples and their stability was studied. The nanoparticles obtained presented a particle size of 340 nm with a positive surface charge of 44 mV and the capability of forming lipoplexes with DNA plasmids was stated. (C) 2013 Elsevier B.V. All rights reserved.

引用

页码：157 / 165

页数：9

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