Performance of optoacoustic and fluorescence imaging in detecting deep-seated fluorescent agents

被引:38
作者
Chen, Zhenyue [1 ]
Dean-Ben, Xose Luis [1 ]
Gottschalk, Sven [1 ]
Razansky, Daniel [1 ,2 ]
机构
[1] Helmholtz Ctr Munich, IBMI, Ingolstadter Landstr 1, D-85764 Neuherberg, Germany
[2] Tech Univ Munich, Fac Med, Ismaninger Str 22, D-81675 Munich, Germany
基金
欧洲研究理事会;
关键词
DIFFUSE OPTICAL TOMOGRAPHY; IN-VIVO; MICROSCOPY; CONTRAST; BRAIN; PROTEINS; DYNAMICS; TISSUES; CELLS;
D O I
10.1364/BOE.9.002229
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Fluorescent contrast agents are widely employed in biomedical research. While many studies have reported deep tissue imaging of fluorescent moieties using either fluorescence-based or absorption-based (optoacoustic) imaging systems, no systematic comparison has been performed regarding the actual performance of these imaging modalities in detecting deep-seated fluorescent agents. Herein, an integrated imager combining epi-fluorescence and volumetric optoacoustic imaging capabilities has been employed in order to evaluate image degradation with depth for several commonly-used near-infrared dyes in both modes. We performed controlled experiments in tissue-mimicking phantoms containing deeply embedded targets filled with different concentrations of Alexa Fluor 700, Alexa Fluor 750, indocyanine green (ICG) and IRDye 800CW. The results are further corroborated by multi- modal imaging of ICG through mouse tissues in vivo. It is shown that optoacoustics consistently provides better sensitivity in differentiating fluorescent targets located at depths beyond 2 mm in turbid tissues, as quantified by evaluating image contrast, signal to noise ratio and spatial resolution performance. (c) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
引用
收藏
页码:2214 / 2224
页数:11
相关论文
共 47 条
[1]  
Ale A, 2012, NAT METHODS, V9, P615, DOI [10.1038/NMETH.2014, 10.1038/nmeth.2014]
[2]   Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography [J].
Berninger, Markus T. ;
Mohajerani, Pouyan ;
Wildgruber, Moritz ;
Beziere, Nicolas ;
Kimm, Melanie A. ;
Ma, Xiaopeng ;
Haller, Bernhard ;
Fleming, Megan J. ;
Vogt, Stephan ;
Anton, Martina ;
Imhoff, Andreas B. ;
Ntziachristos, Vasilis ;
Meier, Reinhard ;
Henning, Tobias D. .
PHOTOACOUSTICS, 2017, 6 :37-47
[3]   Next-generation in vivo optical imaging with short-wave infrared quantum dots [J].
Bruns, Oliver T. ;
Bischof, Thomas S. ;
Harris, Daniel K. ;
Franke, Daniel ;
Shi, Yanxiang ;
Riedemann, Lars ;
Bartelt, Alexander ;
Jaworski, Frank B. ;
Carr, Jessica A. ;
Rowlands, Christopher J. ;
Wilson, Mark W. B. ;
Chen, Ou ;
Wei, He ;
Hwang, Gyu Weon ;
Montana, Daniel M. ;
Coropceanu, Igor ;
Achorn, Odin B. ;
Kloepper, Jonas ;
Heeren, Joerg ;
So, Peter T. C. ;
Fukumura, Dai ;
Jensen, Klavs F. ;
Jain, Rakesh K. ;
Bawendi, Moungi G. .
NATURE BIOMEDICAL ENGINEERING, 2017, 1 (04)
[4]   Multispectral photoacoustic microscopy based on an optical-acoustic objective [J].
Cao, Rui ;
Kilroy, Joseph P. ;
Ning, Bo ;
Wang, Tianxiong ;
Hossack, John A. ;
Hu, Song .
PHOTOACOUSTICS, 2015, 3 (02) :55-59
[5]   Hybrid system for in vivo epifluorescence and 4D optoacoustic imaging [J].
Chen, Zhenyue ;
Dean-Ben, Xose Luis ;
Gottschalk, Sven ;
Razansky, Daniel .
OPTICS LETTERS, 2017, 42 (22) :4577-4580
[6]  
Cranfill PJ, 2016, NAT METHODS, V13, P557, DOI [10.1038/NMETH.3891, 10.1038/nmeth.3891]
[7]   Advanced optoacoustic methods for multiscale imaging of in vivo dynamics [J].
Dean-Ben, X. L. ;
Gottschalk, S. ;
Mc Larney, B. ;
Shoham, S. ;
Razansky, D. .
CHEMICAL SOCIETY REVIEWS, 2017, 46 (08) :2158-2198
[8]   Functional optoacoustic neuro-tomography of calcium fluxes in adult zebrafish brain in vivo [J].
Dean-Ben, X. Luis ;
Gottschalk, Sven ;
Sela, Gali ;
Shoham, Shy ;
Razansky, Daniel .
OPTICS LETTERS, 2017, 42 (05) :959-962
[9]   Light fluence normalization in turbid tissues via temporally unmixed multispectral optoacoustic tomography [J].
Dean-Ben, X. Luis ;
Stiel, Andre C. ;
Jiang, Yuanyuan ;
Ntziachristos, Vasilis ;
Westmeyer, Gil G. ;
Razansky, Daniel .
OPTICS LETTERS, 2015, 40 (20) :4691-4694
[10]   Volumetric Real-Time Tracking of Peripheral Human Vasculature With GPU-Accelerated Three-Dimensional Optoacoustic Tomography [J].
Dean-Ben, X. Luis ;
Ozbek, Ali ;
Razansky, Daniel .
IEEE TRANSACTIONS ON MEDICAL IMAGING, 2013, 32 (11) :2050-2055