Corr4A and VRT325 do not Reduce the Inflammatory Response to P. aeruginosa in Human Cystic Fibrosis Airway Epithelial Cells

被引：15

作者：

Talebian, Laleh ^{[1
]}

Coutermarsh, Bonita ^{[1
]}

Channon, Jacqueline Y. ^{[2
]}

Stanton, Bruce A. ^{[1
]}

机构：

[1] Dartmouth Med Sch, Dept Physiol, Hanover, NH USA

[2] Dartmouth Med Sch, Dept Microbiol & Immunol, Lebanon, NH USA

来源：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2009年 / 23卷 / 1-3期

关键词：

CFTR; P; aeruginosa; Cystic fibrosis; Drug treatment; DF508-CFTR; Cl secretion; NF-KAPPA-B; PSEUDOMONAS-AERUGINOSA; CFTR; SECRETION; EXPRESSION; ACTIVATION; CORRECTORS;

D O I：

10.1159/000204108

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Background: P. aeruginosa chronically colonizes the lung in CF patients and elicits a proinflammatory response. Excessive secretion of IL-6 and IL-8 by CF airway cells in response to P. aeruginosa infection in the CF airway is though to contribute to lung injury. Accordingly, the goal of this study was to test the hypothesis that Corr4a and VRT325, investigational compounds that increase Delta F508-CFTR mediated Cl(-)secretion in human CF airway cells, reduce the pro-inflammatory response to P. aeruginosa. Methods: IL-6 and IL-8 secretion by polarized CF human airway epithelial cells (CFBE41o-) were measured by multiplex analysis, and Delta F508-CFTR Cl- secretion was measured in Ussing chambers. Airway cells were exposed to P. aeruginosa (PAO1 or PA14) and Corr4a or VRT325. Results: Corr4a and VRT325 increased Delta F508-CFTR Cl- secretion but did not reduce either constitutive IL-6 or IL-8 secretion, or IL-6 and IL-8 secretion stimulated by P. aeruginosa (PA14 or PAO1). Conclusions: Corr4a and VRT325 do not reduce the inflammatory response to P. aeruginosa in human cystic fibrosis airway epithelial cells. Copyright (C) 2009 S. Karger AG, Basel

引用

页码：199 / 204

页数：6

共 29 条

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