MicroRNAs in Salivary Exosome as Potential Biomarkers of Aging

被引:120
作者
Machida, Tatsuya [1 ]
Tomofuji, Takaaki [1 ,2 ]
Ekuni, Daisuke [1 ]
Maruyama, Takayuki [3 ]
Yoneda, Toshiki [1 ]
Kawabata, Yuya [1 ]
Mizuno, Hirofumi [1 ]
Miyai, Hisataka [1 ]
Kunitomo, Muneyoshi [1 ]
Morita, Manabu [1 ]
机构
[1] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Prevent Dent, Kita Ku, Okayama 7008558, Japan
[2] Okayama Univ, Sch Dent, Adv Res Ctr Oral & Craniofacial Sci, Kita Ku, Okayama 7008558, Japan
[3] Okayama Univ Hosp, Ctr Innovat Clin Med, Kita Ku, Okayama 7008558, Japan
关键词
microRNA; saliva; exosome; aging; AGE-RELATED-CHANGES; EXPRESSION; PERSPECTIVES; MODULATION; PCR;
D O I
10.3390/ijms160921294
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The aim of this study was to examine whether salivary exosomal miRNAs could be identified as aging biomarkers. Fifteen young healthy volunteers (median age, 21.0 years) and 13 old individuals (median age, 66.0 years) were recruited. Unstimulated whole saliva was collected, salivary exosomes were isolated, and total RNA was extracted. In a microarray, 242 miRNAs were commonly detected in these two mixed samples. Based on the cut-off values of 2- or 0.5-fold changes (FC) and regulatory power for aging process, six candidate miRNAs (miR-24-3p, miR-371a-5p, miR-3175, miR-3162-5p, miR-671-5p, and miR-4667-5p) were selected. After comparing each total RNA obtained by the 15 young and 13 old individuals to validate the FC values using quantitative real-time PCR, miR-24-3p was identified as a novel candidate aging biomarker. This pilot study suggested that salivary exosomal miRNAs could be identified as candidate aging biomarkers. To confirm whether miR-24-3p in salivary exosomes are suitable biomarkers of aging, further validation research is required.
引用
收藏
页码:21294 / 21309
页数:16
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