Sensitive Determination of Bovine Serum Albumin by Flow-Injection Chemiluminescence

被引：0
作者：
Du Jun-jun [1 ]
Wang Hui [1 ]
Ren Shu-wei [2 ]
Zhou Min [1 ]
Liu Ying-ying [1 ]
Liu Yan-ming [1 ]
机构：
[1] Xinyang Normal Univ, Coll Chem & Chem Engn, Xinyang 464000, Peoples R China
[2] Xinyang Cent Hosp, Xinyang 464000, Peoples R China
来源：
SPECTROSCOPY AND SPECTRAL ANALYSIS | 2013年 / 33卷 / 06期
关键词：
Flow-injection; ChemiLuminescence; Bovine serum albumin; Mechanism; IMMUNOASSAY; PROTEIN; COMPLEX; PROBE;
D O I：
10.3964/j.issn.1000-0593(2013)06-1693-04
中图分类号：
O433 [光谱学];
学科分类号：
0703 ; 070302 ;
摘要：
A new, rapid and sensitive method for the determination of bovine serum albumin (BSA) by flow-injection coupled with chemiLuminescence detection was establish, which based on BSA enhancing effect on weak chemiLuminescence system of Luminol-H2O2 in alkaline solution. Under the optimized conditions, linearity for BSA was found in the range of 2.5 x 10(-9)similar to 1. 0 x 10(-6) mol . L-1, with the detection limit of 5.8 x 10(-10) mol . L-1. And the sampling detecting rate was about 112 samples . h(-1). The relative standard deviation(RSD) was found to be 0.8% for 11 replicate determinations of 1.0 x 10(-7) mol . L-1 BSA. The proposed method has been satisfactorily applied to the determination of BSA in the bovine serum samples. The possible mechanism of Luminol-H2O2-BSA was studied by chemiLuminescence spectrum and UV-visible spectrum.
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页码：1693 / 1696
页数：4
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