Differential Expression of Micro-Heterogeneous LewisX-Type Glycans in the Stem Cell Compartment of the Developing Mouse Spinal Cord

被引:13
作者
Karus, Michael [1 ,2 ]
Hennen, Eva [1 ,2 ]
Safina, Dina [1 ,2 ]
Klausmeyer, Alice [1 ,3 ]
Wiese, Stefan [1 ,3 ]
Faissner, Andreas [1 ]
机构
[1] Ruhr Univ Bochum, Dept Cell Morphol & Mol Neurobiol, Bochum, Germany
[2] Ruhr Univ Bochum, Int Grad Sch Neurosci, Bochum, Germany
[3] Ruhr Univ Bochum, Grp Mol Cell Biol, Bochum, Germany
关键词
Complex glycans; Extracellular matrix; Neural stem cells; Stem cell niche; Tenascin-C; Receptor protein tyrosinphosphatase-beta/zeta; NEURAL STEM/PROGENITOR CELLS; CENTRAL-NERVOUS-SYSTEM; TENASCIN-C; NEURITE OUTGROWTH; PROGENITOR CELLS; RADIAL GLIA; PROLIFERATION; PROTEOGLYCAN; CARBOHYDRATE; NEURONS;
D O I
10.1007/s11064-013-1048-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Complex glycan structures and their respective carrier molecules are often expressed in a cell type specific manner. Thus, glycans can be used for the enrichment of specific cell types such as neural precursor cells (NPCs). We have recently shown that the monoclonal antibodies 487(LeX) and 5750(LeX) differentially detect the LewisX (LeX) glycan on NPCs in the developing mouse forebrain. Here, we analysed the staining pattern of both antibodies during late embryonic mouse spinal cord development. At E13.5 both antibodies strongly label the central canal region. Along these lines they detect the LeX glycan primarily on Nestin-positive NPCs at that age. Moreover, we show that spinal cord NPCs cultured as free floating neurospheres display a high immunoreactivity to both antibodies. In that context, we also demonstrate that the 487(LeX) antibody can be used to deplete a subpopulation of neurosphere forming NPCs from a mixed E13.5 spinal cord cell suspension. Towards the end of embryogenesis the overall immunoreactivity to both antibodies increases and the staining appears very diffuse. However, the 5750(LeX) antibody still labels the central canal region. The increase in immunoreactivity correlates with an expression increase of the extracellular matrix molecules Tenascin C and Receptor Protein Tyrosine Phosphatase beta/zeta, two potential LeX carrier proteins. In line with this, immunoprecipitation analyses confirmed Tenascin C as a LeX carrier protein in the embryonic mouse spinal cord. However, the immunoreactivity to both antibodies appears only to be marginally affected in the absence of Tenascin C, arguing against Tenascin C being the major LeX carrier. In conclusion our study gives some novel insights into the complex expression of LeX glycans and potential carrier proteins during the development of the mouse spinal cord.
引用
收藏
页码:1285 / 1294
页数:10
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