An artificial protein-probe hybrid as a responsive probe for ratiometric detection and imaging of hydrogen peroxide in cells

被引:17
作者
Zhang, Xing [1 ,2 ]
Fu, Youxin [2 ]
Qian, Guangren [1 ]
Zhang, Run [2 ]
Xu, Zhi Ping [2 ]
机构
[1] Shanghai Univ, Schaal Environm Sci & Chem Engn, Shanghai 200444, Peoples R China
[2] Univ Queensland, Australian Inst Bioengn & Nanotechnol, St Lucia, Qld 4072, Australia
基金
澳大利亚研究理事会;
关键词
FLUORESCENT-PROBE; SERUM-ALBUMIN; LUMINESCENT; BINDING; BSA;
D O I
10.1039/d0tb00856g
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
0805 ; 080501 ; 080502 ;
摘要
An artificial protein-probe hybrid,Cm-Np-B@BSA, was preparedviahost-guest interactions between hydrogen peroxide (H2O2)-responsiveCm-Np-Bmolecule and bovine serum albumin (BSA). TheCm-Np-B@BSAprobe exhibited high sensitivity and selectivity towards H(2)O(2)under physiological conditions and had excellent biocompatibility, allowing for sensitive ratiometric detection and imaging of endogenous H(2)O(2)in live cells.
引用
收藏
页码:5420 / 5424
页数:5
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