Activation-Induced Cytidine Deaminase Induces DNA Demethylation of Pluripotency Genes in Bovine Differentiated Cells

被引：4
作者：
Moon, Song-Yi [1 ]
Eun, Hye-Ju [1 ]
Baek, Sang-Ki [1 ]
Jin, Sang-Jin [1 ]
Kim, Tae-Suk [1 ,2 ]
Kim, Sung-Woo [3 ]
Seong, Hwan-Hoo [3 ]
Choi, In-Chul [4 ]
Lee, Joon-Hee [1 ,2 ]
机构：
[1] Gyeongsang Natl Univ, Dept Anim Biosci, Coll Agr & Life Sci, Jinju 52828, South Korea
[2] Gyeongsang Natl Univ, Inst Agr & Life Sci, Coll Agr & Life Sci, Jinju, South Korea
[3] Natl Inst Anim Sci, Rural Dev Adm, Anim Genet Resources Stn, Namwon, South Korea
[4] Chungnam Natl Univ, Coll Agr & Life Sci, Div Anim & Dairy Sci, Daejeon, South Korea
来源：
CELLULAR REPROGRAMMING | 2016年 / 18卷 / 05期
基金：
新加坡国家研究基金会;
关键词：
BASE EXCISION-REPAIR; SINGLE-STRANDED-DNA; STEM-CELLS; MAMMALIAN DEVELOPMENT; NUCLEAR TRANSFER; PATERNAL GENOME; METHYLATION; AID; 5-METHYLCYTOSINE; 5-HYDROXYMETHYLCYTOSINE;
D O I：
10.1089/cell.2015.0076
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Activation-induced cytidine deaminase (AID) is the only enzyme that has been suggested as a putative DNA demethylase in mammals. However, very little is known about AID function as DNA demethylase of bovine differentiated cells toward pluripotent state. To investigate the effect of AID on DNA demethylation, bovine AID complementary DNAs were transfected into bovine differentiated cells, which were mostly methylated in the promoter regions of pluripotency genes. As a result, AID-transfected bovine cells started to transform into colonies at day 19 of transfection. The colonies derived from the transfected cells showed positive alkaline phosphatase (AP) staining and expression of pluripotency genes (OCT-3/4, NANOG, SOX2) and pluripotency-related antigens (SSEA-4, TRA1-60, TRA1-81), which have been widely used to characterize human embryonic stem cells. In particular, the levels of OCT-3/4 and NANOG expression were significantly increased in the AID-transfected cells when compared with the control and empty vector-transfected cells (p < 0.05). Finally, DNA demethylation in the promoter regions of pluripotency genes (OCT-3/4, NANOG) was significantly increased compared with the control (p < 0.05). These results demonstrate that the induction of the AID gene into bovine differentiated cells improves DNA demethylation and expression of pluripotency genes.
引用
收藏
页码：298 / 308
页数：11
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