Enrichment and Purification of Total Flavonoid C-Glycosides from Abrus mollis Extracts with Macroporous Resins

In the present study, the performance and separation characteristics of six macroporous resins for the enrichment and purification of total flavonoid C-glycosides (vicenin-2, 1; isoschaftoside, 2; schaftoside, 3) from Abrus mollis extracts have been evaluated. The adsorption and desorption properties of total flavonoid C-glycosides were studied on macroporous resins, including HPD-100, HPD-450, AB-8, HPD-600, DA-201, and HPD-417. According to the results, HPD-100 resin showed the best adsorption and desorption capacity for these three flavonoid C-glycosides among six resins. Adsorption isotherms were constructed on HPD-100 resin and fitted well to the Langmuir (R-2 > 0.99) and Freundlich (R-2 > 0.89, 0.1 < 1/n < 0.5) models. After the treatment with gradient elution on HPD-100 resin packed chromatography column, the contents of the flavonoid C-glycosides (1, 2, and 3) increased from 2.05, 2.90, and 2.30% in the extracts to 14.3, 21.9, and 15.1%, respectively, in the product. The recoveries of compounds 1, 2, and 3 were 83.8, 90.3, and 78.7%, respectively.