Comparative study on enzymatic digestibility of acid-pretreated poplar and larch based on a comprehensive analysis of the lignin-derived recalcitrance

被引：77

作者：

Jia, Yuan ^{[1
]}

Yang, Chundong ^{[1
]}

Shen, Buzhen ^{[1
]}

Ling, Zhe ^{[1
]}

Huang, Caoxing ^{[1
]}

Li, Xin ^{[1
,2
]}

Lai, Chenhuan ^{[1
,2
]}

Yong, Qiang ^{[1
,2
]}

机构：

[1] Nanjing Forestry Univ, Coll Chem Engn, Jiangsu Coinnovat Ctr Efficient Proc & Utilizat F, Nanjing 210037, Peoples R China

[2] Nanjing Forestry Univ, Minist Educ, Key Lab Forestry Genet & Biotechnol, Nanjing 210037, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2021年 / 319卷

基金：

中国国家自然科学基金;

关键词：

Acid pretreatment; Poplar; Larch; Enzymatic hydrolysis; Lignin inhibition; HYDROLYSIS; CELLULOSE; SACCHARIFICATION; DELIGNIFICATION; SELECTIVITY; INHIBITION; ENHANCE; BIOMASS; IMPACT; L;

D O I：

10.1016/j.biortech.2020.124225

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Enzymatic digestibility of an acid-pretreated poplar (AP, 42.9%) was superior to that of a similarly acid pretreated larch (AL, 12.5%). Effects of lignin-related recalcitrance on enzymatic hydrolysis were comprehensively investigated by disrupting the two predominant lignin fractions present in acid-pretreated material (extractable lignin and bulk lignin). Lignin removal and bovine serum albumin (BSA) addition were performed to estimate the relative contributions of lignin towards physical blocking and enzyme binding on enzymatic hydrolysis. The lignin physical blocking played a more significant role in limiting the enzymatic hydrolysis of AL. BSA addition improved enzymatic hydrolysis of AP more significantly than AL. Moreover, the effects of lignin embedded in the lignocellulosic matrix on enzyme non-productive binding were compared with the isolated lignin. It indicated that the lignin distribution would influence the lignin effects on enzyme non-productive binding during enzymatic hydrolysis. Results will give insights towards improvement of enzymatic hydrolysis on acid-pretreated woody biomass.

引用

页数：9

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