The Effect of Insulin Treatment on Rac1 Expression in Diabetic Kidney

被引：5
|
作者：
Gumustekin, Mukaddes [1 ]
Micili, Serap Cilaker [2 ]
Arici, Mualla Aylin [1 ]
Karaman, Meral [3 ]
Guneli, Mehmet Ensari [3 ]
Tekmen, Isil [2 ]
机构：
[1] Dokuz Eylul Univ, Sch Med, Dept Pharmacol, Izmir, Turkey
[2] Dokuz Eylul Univ, Sch Med, Dept Histol & Embryol, Izmir, Turkey
[3] Dokuz Eylul Univ, Sch Med, Dept Lab Anim Sci, Izmir, Turkey
来源：
RENAL FAILURE | 2013年 / 35卷 / 03期
关键词：
apoptosis; caspase-3; diabetic nephropathy; insulin; Rac1; TUBULAR EPITHELIAL-CELLS; NADPH OXIDASE; OXIDATIVE STRESS; APOPTOSIS; MELATONIN; NEPHROPATHY; PROTECTS; GLUCOSE; GTPASE; DAMAGE;
D O I：
10.3109/0886022X.2013.764256
中图分类号：
R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];
学科分类号：
1002 ; 100201 ;
摘要：
This study was designed to evaluate the renoprotective effect of insulin on diabetic nephropathy through Rac1 inhibition. Twenty Wistar rats were divided into three groups: control (C), diabetic (D), and insulin-treated diabetic (D + I). Diabetes was induced by a single streptozotocin (STZ) injection (45 mg/kg i.p.) in adult male rats. Diabetic animals were treated subcutaneously with insulin (6 U/kg), or saline once a day for 8 weeks. Age-matched control rats received only saline. The kidney tissue samples were analyzed by immunohistochemical staining for Rac1 and cleaved caspase-3 expressions and using the TUNEL method for determining apoptotic cells. Diabetes increased the number of TUNEL (+) cells and cleaved caspase-3 and Rac1 expression levels in kidney. Administration of insulin for 8 weeks reduced Rac1 expression and ameliorated histopathological changes in kidney of STZ-induced diabetes model. These results may suggest that the renoprotective effect of insulin at least partly results from inhibition of Rac1 overexpression.
引用
收藏
页码：396 / 402
页数：7
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