The Effect of Insulin Treatment on Rac1 Expression in Diabetic Kidney

被引：6

作者：

Gumustekin, Mukaddes ^{[1
]}

Micili, Serap Cilaker ^{[2
]}

Arici, Mualla Aylin ^{[1
]}

Karaman, Meral ^{[3
]}

Guneli, Mehmet Ensari ^{[3
]}

Tekmen, Isil ^{[2
]}

机构：

[1] Dokuz Eylul Univ, Sch Med, Dept Pharmacol, Izmir, Turkey

[2] Dokuz Eylul Univ, Sch Med, Dept Histol & Embryol, Izmir, Turkey

[3] Dokuz Eylul Univ, Sch Med, Dept Lab Anim Sci, Izmir, Turkey

来源：

RENAL FAILURE | 2013年 / 35卷 / 03期

关键词：

apoptosis; caspase-3; diabetic nephropathy; insulin; Rac1; TUBULAR EPITHELIAL-CELLS; NADPH OXIDASE; OXIDATIVE STRESS; APOPTOSIS; MELATONIN; NEPHROPATHY; PROTECTS; GLUCOSE; GTPASE; DAMAGE;

D O I：

10.3109/0886022X.2013.764256

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

This study was designed to evaluate the renoprotective effect of insulin on diabetic nephropathy through Rac1 inhibition. Twenty Wistar rats were divided into three groups: control (C), diabetic (D), and insulin-treated diabetic (D + I). Diabetes was induced by a single streptozotocin (STZ) injection (45 mg/kg i.p.) in adult male rats. Diabetic animals were treated subcutaneously with insulin (6 U/kg), or saline once a day for 8 weeks. Age-matched control rats received only saline. The kidney tissue samples were analyzed by immunohistochemical staining for Rac1 and cleaved caspase-3 expressions and using the TUNEL method for determining apoptotic cells. Diabetes increased the number of TUNEL (+) cells and cleaved caspase-3 and Rac1 expression levels in kidney. Administration of insulin for 8 weeks reduced Rac1 expression and ameliorated histopathological changes in kidney of STZ-induced diabetes model. These results may suggest that the renoprotective effect of insulin at least partly results from inhibition of Rac1 overexpression.

引用

页码：396 / 402

页数：7

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