Cloning and sequencing of a full-length sea bream (Sparus aurata) beta-actin cDNA

被引:31
作者
Santos, CRA
Power, DM
Kille, P
Llewellyn, L
Ramsurn, V
Wigham, T
Sweeney, GE
机构
[1] UNIV ALGARVE,CCMAR,P-8000 FARO,PORTUGAL
[2] UNIV WALES COLL CARDIFF,SCH PURE & APPL BIOL,CARDIFF CF1 3NS,S GLAM,WALES
[3] UNIV WALES COLL CARDIFF,SCH MOL & MED BIOSCI,CARDIFF CF1 3NS,S GLAM,WALES
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1997年 / 117卷 / 02期
关键词
beta-actin cDNA; conservation; evolution; sea bream (Sparus aurata); teleost;
D O I
10.1016/S0305-0491(96)00328-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A full-length cDNA clone encoding beta-actin (beta-actin) was isolated from a sea bream (Sparus aurata) liver cDNA library. Sequencing of this clone reveals an open reading frame encoding a 375 amino acid protein that shares a high degree of conservation to other known actins. The sea bream beta-actin sequence showed 98% identity to carp and human beta-actin and 95% and 94% identity to sea squirt and Dictyostelium cytoplasmic actins, respectively. (C) 1997 Elsevier Science Inc.
引用
收藏
页码:185 / 189
页数:5
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