EFFECT OF VITAMIN C ON GLUCOSE METABOLIZING ENZYMES IN CYCLOPHOSPHAMIDE, METHOTREXATE AND 5-FLUOROURACIL TREATED FIBROSARCOMA BEARING RATS

The effect of vitamin C on carbohydrate metabolizing enzymes in cyclophosphamide, methotrexate and 5-fluorouracil treated fibrosarcoma bearing rats was evaluated in the present study, since the effect of vitamin Care beneficial on the abnormalities induced by cyclophosphamide, methotrexate and 5-fluorouracil. The fibrosarcoma cell lines induced rats were treated with cyclophosphamide, methotrexate and 5-fluorouracil and vitamin C (cyclophosphamide 10mg/kg, methotrexate 1mg/kg, 5-fluorouracil 10mg/kg and vitamin C 200mg/kg body weight) individually and in combination for 120 days. Since some definite correlation exists between tumor progression and the activities of glycolytic and gluconeogenic enzymes, assessment of alterations in the activity was considered as successful markers of diagnosis. The untreated as well as cyclophosphamide, methotrexate and 5-fluorouracil treated fibrosarcoma bearing rats showed significantly decrease in the activity of glycolytic enzymes and increasing gluconeogenic enzyme activities were found to be normalized by co-administration of vitamin C. These results suggested that the abnormalities of cyclophosphamide, methotrexate and 5-fluorouracil were corrected by co-administration of vitamin C in tumor stress condition.