Interleukin-10 spot-forming cells as a novel biomarker of chronic graft-versus-host disease

被引：8

作者：

Hirayama, Masahiro ^{[1
]}

Azuma, Eiichi ^{[2
]}

Nakagawa-Nakazawa, Atsuko ^{[3
]}

Kumamoto, Tadashi ^{[1
]}

Iwamoto, Shotaro ^{[1
]}

Amano, Keishiro ^{[1
]}

Tamaki, Shigehisa ^{[4
]}

Usui, Eiji ^{[4
]}

Komada, Yoshihiro ^{[1
]}

机构：

[1] Mie Univ, Dept Pediat, Grad Sch Med, Tsu, Mie 514, Japan

[2] Mie Univ, Dept Cell Transplantat, Grad Sch Med, Tsu, Mie 514, Japan

[3] Natl Ctr Child Hlth & Dev, Dept Pathol, Setagaya Ku, Tokyo, Japan

[4] Ise Red Cross Hosp, Dept Internal Med, Ise, Mie, Japan

来源：

HAEMATOLOGICA-THE HEMATOLOGY JOURNAL | 2013年 / 98卷 / 01期

关键词：

CONSENSUS DEVELOPMENT PROJECT; WORKING GROUP-REPORT; REGULATORY T-CELLS; PLASMACYTOID DENDRITIC CELLS; PERIPHERAL-BLOOD; CLINICAL-TRIALS; EXTRACORPOREAL PHOTOCHEMOTHERAPY; CHRONIC GVHD; BONE-MARROW; B-CELLS;

D O I：

10.3324/haematol.2012.069815

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Although there are National Institutes of Health consensus criteria for the global assessment of chronic graft-versus- host disease, no validated biomarkers have been established for this disease. Furthermore, whereas the role of T cells, B cells, and dendritic cells in chronic graft-versus-host disease has been established, the contribution of monocytes has not been clearly addressed. Using an enzyme-linked immunospot assay, we measured the spot-forming cells for interferon-gamma, interleukin-4, interleukin-10, and interleukin-17 in unstimulated peripheral blood of patients following allogeneic hematopoietic stem cell transplantation. Other immunological examinations, including skin biopsy, were also done. Fifty-seven patients were enrolled. Interleukin-10 spot-forming cells were evaluable for therapeutic monitoring in 16 patients with chronic graft-versus-host disease. The number of interleukin-10 spot-forming cells in patients with active chronic graft-versus-host disease was significantly higher than the number in those with no or inactive chronic graft-versus-host disease. Interleukin-10 was predominantly produced by monocytes. CD29 expression on monocytes in patients with active chronic graft-versus-host disease was elevated. The level of plasma fibronectin, a ligand of CD29, correlated with the number of interleukin-10 spot-forming cells. Immunohistochemical analysis of the skin in active chronic graft-versus-host disease showed that infiltrating CD29(+) monocytes might produce interleukin-10. A novel biomarker, interleukin-10 spot-forming cells, shows promise as both a diagnostic and prognostic indicator for chronic graft-versus-host disease, and may allow for early intervention prior to the onset of the disease. Measurement of interleukin-10 spot-forming cells would be helpful in clinical trials and in patients' management. (UMIN-CTR UMIN000006733; www.umin.ac.jp/ctr/index/htm) (C) 2013 Ferrata Storti Foundation. This is an open-access paper. doi:10.3324/haematol.2012.069815

引用

页码：41 / 49

页数：9

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