DNA damage in BV-2 cells: An important supplement to the neurotoxicity of CdTe quantum dots

被引:35
作者
He, Keyu [1 ,2 ,3 ,4 ]
Liang, Xue [1 ,2 ,3 ,4 ]
Wei, Tingting [1 ,2 ,3 ,4 ]
Liu, Na [1 ,2 ,3 ,4 ]
Wang, Yan [1 ,2 ,3 ,4 ]
Zou, Linyue [1 ,2 ,3 ,4 ]
Lu, Jie [1 ,2 ,3 ,4 ]
Yao, Ying [1 ,2 ,3 ,4 ]
Kong, Lu [1 ,2 ,3 ,4 ]
Zhang, Ting [1 ,2 ,3 ,4 ]
Xue, Yuying [1 ,2 ,3 ,4 ]
Wu, Tianshu [1 ,2 ,3 ,4 ]
Tang, Meng [1 ,2 ,3 ,4 ]
机构
[1] Southeast Univ, Key Lab Environm Med & Engn, Minist Educ, Nanjing 210009, Jiangsu, Peoples R China
[2] Southeast Univ, Sch Publ Hlth, Nanjing 210009, Jiangsu, Peoples R China
[3] Southeast Univ, Collaborat Innovat Ctr Suzhou Nano Sci & Technol, Nanjing 210009, Jiangsu, Peoples R China
[4] Southeast Univ, Jiangsu Key Lab Biomat & Devices, Nanjing 210009, Jiangsu, Peoples R China
关键词
BV-2; cells; DNA damage; glutathione; in vitro study; microglia; neurotoxicology; quantum dots; ROS; NANOCRYSTALS; MICROGLIA; LIGANDS;
D O I
10.1002/jat.3745
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
Microglial cells are resident immune cells in the central nervous system. Activation of microglia as induced by CdTe quantum dots (QDs) can trigger damage to neurons. To quantify the intracellular QDs, we monitored the intracellular Cd concentration in the QD-exposed mouse microglial cells (BV-2 cell line). The extent of cell injury at different times correlated with the Cd concentration in cells at that time. In addition to Cd ion detection, we also monitored the intracellular fluorescence of the QDs. More QDs accumulated in the nucleus than in the cytoplasm. Comet assays confirmed that QDs induce DNA damage. However, DNA cannot interact with QDs, so the DNA damage was not caused by CdTe QDs adducts to DNA but by the increase of the Cd ion concentration and the secondary oxidative damage. In addition to DNA damage, biofilm injury and endogenous reduced glutathione depletion were also apparent in QD-exposed BV-2 cells. These changes can be prevented or even reversed by exogenous reduced glutathione administration.
引用
收藏
页码:525 / 539
页数:15
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