Ammonium fluoride as a mobile phase additive in aqueous normal phase chromatography

被引：34

作者：

Pesek, Joseph J. ^{[1
]}

Matyska, Maria T. ^{[1
]}

机构：

[1] San Jose State Univ, Dept Chem, San Jose, CA 95192 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2015年 / 1401卷

关键词：

Silica hydride; Mass spectrometry; Mobile phase additive; SILICA-HYDRIDE; STATIONARY PHASES; HYDROPHILIC METABOLITES; LIQUID-CHROMATOGRAPHY; POLAR; SEPARATION; RETENTION;

D O I：

10.1016/j.chroma.2015.05.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of ammonium fluoride as a mobile phase additive in aqueous normal phase chromatography with silica hydride-based stationary phases and mass spectrometry detection is evaluated. Retention times, peak shape, efficiency and peak intensity are compared to the more standard additives formic acid and ammonium formate. The test solutes were NAD, 3-hydroxyglutaric acid, alpha-ketogiutaric acid, p-aminohippuric acid, AMP, ATP, aconitic acid, threonine, N-acetyl carnitine, and 3-methyladipic acid. The column parameters are assessed in both the positive and negative ion detection modes. Ammonium fluoride is potentially an aggressive mobile phase additive that could have detrimental effects on column lifetime. Column reproducibility is measured and the effects of switching between different additives are also tested. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：69 / 74

页数：6

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