Identification and comparative analysis of the large subunit mitochondrial ribosomal proteins of Neurospora crassa

被引:9
作者
Gan, X
Arita, K
Isono, S
Kitakawa, M [1 ]
Yoshin, K
Yonezawa, K
Kato, A
Inoue, H
Isono, K
机构
[1] Kobe Univ, Fac Sci, Dept Biol, Kobe, Hyogo 6578501, Japan
[2] Kobe Univ, Biosignal Res Ctr, Kobe, Hyogo 6578501, Japan
[3] Kobe Univ, Grad Sch Sci & Technol, Kobe, Hyogo 6578501, Japan
[4] Saitama Univ, Fac Sci, Dept Regulat Biol, Urawa, Saitama, Japan
[5] Hu Bei Univ, Fac Life Sci, Hubei, Peoples R China
关键词
mitochondrial ribosomal protein; Neurospora crassa; Saccharomyces cerevisiae; mass spectrometry; MHRI; L7/12;
D O I
10.1111/j.1574-6968.2005.00024.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The mitochondrial ribosome (mitoribosome) has highly evolved from its putative prokaryotic ancestor and varies considerably from one organism to another. To gain further insights into its structural and evolutionary characteristics, we have purified and identified individual mitochondrial ribosomal proteins of Neurospora crassa by mass spectrometry and compared them with those of the budding yeast Saccharomyces cerevisiae. Most of the mitochondrial ribosomal proteins of the two, fungi are well conserved with each other, although the degree of conservation varies to a large extent. One of the N. crassa mitochondrial ribosomal proteins was found to be homologous to yeast Mhrlp that is involved in homologous DNA recombination and genome maintenance in yeast mitochondria.
引用
收藏
页码:157 / 164
页数:8
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