To investigate the acceptance of automated breast ultrasound (ABUS), mammography (MAM) and handheld ultrasound (HHUS) among Chinese female subjects, and explore how the breast screening experience can be improved. The satisfaction and pain score of 652 female subjects who performed ABUS, MAM and HHUS examinations were collected via questionnaires. The pain score difference between the screening methods was determined by the Kruskal-Wallis test. Spearman correlation analyses were also performed to assess the association between pain scores across the screening methods and subject characteristics. Furthermore, we compared the diagnostic consistency of ABUS with between MAM and HHUS. Amongst the three breast screening methods investigated, MAM was rated with the highest pain score, significantly greater than ABUS and HHUS. 92.9% and 94.7% of subjects preferred ABUS and HHUS examination, respectively, while only 41.0% preferred MAM. MAM pain soores were negatively correlated to age, bra size, and menopausal status, and also positively correlated to the first breastfeeding age and breast density. ABUS pain scores were negatively correlated to BMI and bra size, and also positively correlated with the cumulative lactation time. Finally, HHUS pain scores were found to be negatively correlated with menarche age, lactation times and the cumulative lactation time, and positively correlated to abortion times and menopausal status. The Cohen's kappa coefficient revealed significant agreement between ABUS and MAM, and between ABUS and HHUS. The results show that women have a better acceptance of ABUS than MAM.