The mechanism of force transmission at bacterial focal adhesion complexes

被引：94

作者：

Faure, Laura M. ^{[1
]}

Fiche, Jean-Bernard ^{[2
]}

Espinosa, Leon ^{[1
]}

Ducret, Adrien ^{[1
,3
]}

Anantharaman, Vivek ^{[4
]}

Luciano, Jennifer ^{[1
]}

Lhospice, Sebastien ^{[1
]}

Islam, Salim T. ^{[1
]}

Treguier, Julie ^{[1
]}

Sotes, Melanie ^{[1
]}

Kuru, Erkin ^{[5
]}

Van Nieuwenhze, Michael S. ^{[6
]}

Brun, Yves V. ^{[3
]}

Theodoly, Olivier ^{[7
]}

Aravind, L.

Nollmann, Marcelo ^{[2
]}

Mignot, Tam ^{[1
]}

机构：

[1] Aix Marseille Univ, CNRS, Inst Microbiol Mediterranee, Lab Chim Bacterienne,UMR7283, F-13009 Marseille, France

[2] Univ Montpellier, INSERM, CNRS, Ctr Biochim Struct,UMR5048,U1054, 29 Rue Navacelles, F-34090 Montpellier, France

[3] Indiana Univ, Dept Biol, Bloomington, IN 47405 USA

[4] NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20892 USA

[5] Indiana Univ, Interdisciplinary Biochem Program, Bloomington, IN 47405 USA

[6] Indiana Univ, Dept Chem, Bloomington, IN 47405 USA

[7] Aix Marseille Univ, INSERM, Adhes & Inflammat Lab, U1067,UMR7333, F-13288 Marseille, France

来源：

NATURE | 2016年 / 539卷 / 7630期

基金：

欧洲研究理事会; 加拿大健康研究院;

关键词：

HIDDEN MARKOV MODEL; MYXOCOCCUS-XANTHUS; GLIDING MOTILITY; ACTIN CYTOSKELETON; SIGNAL; TOPOLOGY; ACCURATE;

D O I：

10.1038/nature20121

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Various rod-shaped bacteria mysteriously glide on surfaces in the absence of appendages such as flagella or pili. In the deltaproteobacterium Myxococcus xanthus, a putative gliding motility machinery (the Agl-Glt complex) localizes to so-called focal adhesion sites (FASs) that form stationary contact points with the underlying surface. Here we show that the Agl-Glt machinery contains an inner-membrane motor complex that moves intracellularly along a right-handed helical path; when the machinery becomes stationary at FASs, the motor complex powers a left-handed rotation of the cell around its long axis. At FASs, force transmission requires cyclic interactions between the molecular motor and the adhesion proteins of the outer membrane via a periplasmic interaction platform, which presumably involves contractile activity of motor components and possible interactions with peptidoglycan. Our results provide a molecular model of bacterial gliding motility.

引用

页码：530 / +

页数：20

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