Phylogenetic and genetic variation analyses of porcine circovirus type 2 isolated from China

被引：51

作者：

Yang, S. ^{[1
,2
]}

Yin, S. ^{[1
,2
]}

Shang, Y. ^{[1
,2
]}

Liu, B. ^{[1
,2
]}

Yuan, L. ^{[1
,2
]}

Khan, M. U. Zafar ^{[1
,2
]}

Liu, X. ^{[1
,2
]}

Cai, J. ^{[1
,2
]}

机构：

[1] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Natl Foot & Mouth Dis Reference Lab, Lanzhou, Gansu, Peoples R China

[2] Jiangsu Coinnovat Ctr Prevent & Control Anim Infe, Yangzhou, Peoples R China

来源：

TRANSBOUNDARY AND EMERGING DISEASES | 2018年 / 65卷 / 02期

基金：

中国国家自然科学基金;

关键词：

PCV2d; phylogeny; porcine circovirus type 2; selection pressure; AMINO-ACID MUTATIONS; CAPSID PROTEIN; NUCLEOTIDE-SEQUENCE; UNITED-STATES; PCV2; IDENTIFICATION; EVOLUTION; EPITOPES; DISEASE; PIGS;

D O I：

10.1111/tbed.12768

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Porcine circovirus type 2 (PCV2) is a causative agent of PCV2-associated disease, which is a growing problem in the swine industry worldwide. High nucleotide substitution occurs in the capsid (Cap) gene of PCV2, which allows the continuous evolution and the emergence of novel PCV2 strains. In this study, we sequenced 24 Chinese PCV2 strains collected from healthy and diseased pigs between 2013 and 2015. Analyses of the genome, Cap and phylogeny classified the 24 Chinese PCV2 strains as PCV-2a (four of 24), PCV-2b (five of 24) and PCV-2d (15 of 24). All strains shared 89.5%-100% and 87.2%-100% identities with the nucleotide and amino acid (aa) sequences of Cap, respectively. Selection pressure analysis showed that five sites at the epitope regions in Cap were under positive selection. Further analysis by Jameson-Wolf antigenic index indicated that aa substitutions occurring at the epitope regions contributed to the antigenic alterations of the different PCV2 strains. High genetic variation and genotype shift to PCV2d occurred in recent years, and different genotypes coexisted in Chinese pig herds. The data provide evidence for the increased genetic diversity and insights into the molecular epidemiology of PCV2.

引用

页码：e383 / e392

页数：10

共 42 条

[1] Modification of PCV-2 virulence by substitution of the genogroup motif of the capsid protein [J].