Immunoproteomics of outer membrane proteins and extracellular proteins of Shigella flexneri 2a 2457T

被引：57
作者：
Ying, TY
Wang, HL
Li, MZ
Wang, JJ
Wang, J
Shi, ZX
Feng, EL
Liu, XK
Su, GF
Wei, KH
Zhang, XM
Huang, PT
Huang, LY
机构：
[1] Beijing Inst Biotechnol, State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China
[2] Beijing Inst Pharmaceut Chem, Beijing, Peoples R China
[3] Natl Ctr Biomed Analysis, Beijing, Peoples R China
来源：
PROTEOMICS | 2005年 / 5卷 / 18期
关键词：
extracellular proteins; immunoproteomics; outer membrane proteins; Shigella flexneri 2a 2457T; two-dimensional gel electrophoresis;
D O I：
10.1002/pmic.200401326
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Shigella flexneri 2a is an important pathogen causing bacillary dysentery in humans. In order to investigate any potential vaccine candidate proteins present in outer membrane proteins (OMPs) and extracellular proteins of S. flexneri 2a 2457T, we use the proteome mapping and database analyzing techniques. A subproteome map and database of OMPs were established first. One hundred and nine of the total 126 marked spots were cut out and processed to MALDI-TOF-MS and PMF. Eighty-seven spots were identified and they represented 55 OMP entries. Furthermore, immunoproteomics analysis of OMPs and extracellular proteins were performed. Total of 34 immunoreactive spots were identified, in which 22 and 12 were from OMPs and extracellular proteins, respectively. Eight novel antigens were found and some of these antigens may be potential vaccine candidate proteins. These results are useful for future studying of pathogenicity, vaccine, and novel antibacterial drugs. Maps and tables of all identified proteins are available on the Internet at www.proteomics.com.cn.
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收藏
页码：4777 / 4793
页数：17
相关论文
共 38 条
[1]   Epidemiology of postshigellosis persistent diarrhea in young children [J].
Ahmed, F ;
Ansaruzzaman, M ;
Haque, E ;
Rao, MR ;
Clemens, JD .
PEDIATRIC INFECTIOUS DISEASE JOURNAL, 2001, 20 (05) :525-530
[2]   Genome-based bioinformatic selection of chromosomal Bacillus anthracis putative vaccine candidates coupled with proteomic identification of surface-associated antigens [J].
Ariel, N ;
Zvi, A ;
Makarova, KS ;
Chitlaru, T ;
Elhanany, E ;
Velan, B ;
Cohen, S ;
Friedlander, AM ;
Shafferman, A .
INFECTION AND IMMUNITY, 2003, 71 (08) :4563-4579
[3]   Mapping of Chlamydia trachomatis proteins by immobiline-polyacrylamide two-dimensional electrophoresis: Spot identification by N-terminal sequencing and immunoblotting [J].
Bini, L ;
SanchezCampillo, M ;
Santucci, A ;
Magi, B ;
Marzocchi, B ;
Comanducci, M ;
Christiansen, G ;
Birkelund, S ;
Cevenini, R ;
Vretou, E ;
Ratti, G ;
Pallini, V .
ELECTROPHORESIS, 1996, 17 (01) :185-190
[4]   Identification of a Treponema denticola OppA homologue that binds host proteins present in the subgingival environment [J].
Fenno, JC ;
Tamura, M ;
Hannam, PM ;
Wong, GWK ;
Chan, RA ;
McBride, BC .
INFECTION AND IMMUNITY, 2000, 68 (04) :1884-1892
[5]   ISOLATION OF INTRACELLULAR MEMBRANES BY MEANS OF SODIUM-CARBONATE TREATMENT - APPLICATION TO ENDOPLASMIC-RETICULUM [J].
FUJIKI, Y ;
HUBBARD, AL ;
FOWLER, S ;
LAZAROW, PB .
JOURNAL OF CELL BIOLOGY, 1982, 93 (01) :97-102
[6]  
Haas G, 2002, PROTEOMICS, V2, P313, DOI 10.1002/1615-9861(200203)2:3<313::AID-PROT313>3.0.CO
[7]  
2-7
[8]   PERIPLASMIC PROTEIN ASSOCIATED WITH THE OLIGOPEPTIDE PERMEASES OF SALMONELLA-TYPHIMURIUM AND ESCHERICHIA-COLI [J].
HIGGINS, CF ;
HARDIE, MM .
JOURNAL OF BACTERIOLOGY, 1983, 155 (03) :1434-1438
[9]   POLYPEPTIDE CARTOGRAPHY OF SPIROPLASMA TAIWANENSE [J].
HUMPHERYSMITH, I ;
GUYONNET, F ;
CHASTEL, C .
ELECTROPHORESIS, 1994, 15 (8-9) :1212-1217
[10]   Outer membrane protein A (OmpA): a new pathogen-associated molecular pattern that interacts with antigen presenting cells-impact on vaccine strategies [J].
Jeannin, P ;
Magistrelli, G ;
Goetsch, L ;
Haeuw, JF ;
Thieblemont, N ;
Bonnefoy, JY ;
Delneste, Y .
VACCINE, 2002, 20 :A23-A27
1234