Quantitative production of 2-acetamido-2-deoxy-D-glucose from crystalline chitin by bacterial chitinase

被引：68

作者：

Pichyangkura, R ^{[1
]}

Kudan, S

Kuttiyawong, K

Sukwattanasinitt, M

Aiba, S

机构：

[1] Chulalongkorn Univ, Fac Sci, Dept Biochem, Bangkok 10330, Thailand

[2] Chulalongkorn Univ, Fac Sci, Dept Chem, Ctr Bioact Cpds, Bangkok 10330, Thailand

[3] Natl Inst Adv Ind Sci & Technol, Special Div Human Life Technol, Osaka 5638577, Japan

来源：

CARBOHYDRATE RESEARCH | 2002年 / 337卷 / 06期

关键词：

chitin; chitinase; N-acetyl-D-glucosamine; 2-acetamido-2-deoxy-D-glucose; Bacillus licheniformis; Burkholderia cepacia;

D O I：

10.1016/S0008-6215(02)00007-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Finely powdered alpha- and beta-chitin can be completely hydrolyzed with chitinase (EC 3.2.1.14) and beta-N-acetylhexosaminidase (EC 3.2.1.52) for the production of 2-acetamido-2-deoxy-D-glucose (GlcNAc). Crude chitinase from Burkholderia cepacia TU09 and Bacillus licheniformis SK-1 were used to digest alpha- and beta-chitin powder. Chitinase from B. cepacia TU09 produced GlcNAc in greater than 85% yield from beta- and alpha-chitin within 1 and 7 days, respectively. B. licheniformis SK-1 chitinase completely hydrolyzed P-chitin within 6 days, giving a final GlcNAc yield of 75%, along with 20% of chitobiose. However, only a 41% yield of GlcNAc was achieved from digesting a-chitin with B. licheniformis SK-1 chitinase. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：557 / 559

页数：3

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