Direct determination of p-hydroxymethamphetamine glucuronide in human urine by high-performance liquid chromatography

被引：1
作者：
Nakajima, R
Morishita, M
Aiso, S
Yajima, T
机构：
[1] Keio Univ, Sch Med, Shinjuku Ku, Tokyo 1608582, Japan
[2] Toho Univ, Fac Pharmaceut Sci, Chiba 2748510, Japan
来源：
CHEMICAL & PHARMACEUTICAL BULLETIN | 2006年 / 54卷 / 04期
关键词：
p-hydroxymethamphetamine glucuronide; ion pair reagent; solid-phase extraction; HPLC; urine sample;
D O I：
10.1248/cpb.54.493
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
High-performance liquid chromatography (HPLC) with UV detection for the simultaneous determination of the free form of p-hydroxymethamphetamine (p-OHMA) and its metabolite, glucuronide (p-OHMAG) was accomplished for the first time. We achieved this by employing 1) an ion pair reagent for retention of sample to a solid-phase extraction (SPE) cartridge, Sep-Pak (R) Light C18 and 2) a simple two-step stepwise elution technique for subsequent ion pair RP-HPLC. The proposed method was optimized for resolution of p-OHMAG, p-OHMA and MA. The method was successfully applied to urine samples collected from MA abusers.
引用
收藏
页码：493 / 495
页数：3
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