Efficacy of evaluation of rooster sperm morphology using different staining methods

被引：66

作者：

Lukaszewicz, E. ^{[1
]}

Jerysz, A. ^{[1
]}

Partyka, A. ^{[1
]}

Siudzinska, A. ^{[1
]}

机构：

[1] Wroclaw Univ Environm & Life Sci, Dept Poultry Breeding, PL-51630 Wroclaw, Poland

来源：

RESEARCH IN VETERINARY SCIENCE | 2008年 / 85卷 / 03期

关键词：

Fowl semen; Eosin-nigrosin stains; Sperm morphology;

D O I：

10.1016/j.rvsc.2008.03.010

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

This work focused on inexpensive methods of evaluation fowl sperm morphology, based on eosin-nigrosin smears, which can determine disorders in spermatogenesis and can be recommended for evaluating the fertilising potency and selecting males in flocks reproduced by artificial insemination. Four fowl breeds (Black Minorca, Italian Partridge, Forwerk and Greenleg Partridge) were used to determine the efficacy of sperm morphology evaluation using four eosin-nigrosin staining methods (according to Blom, Bakst and Cecil, Morisson, Jaskowski) and three examiners of different experience (high, medium, novice). There were significant (P <= 0.01) differences in sperm morphology between Blom's staining method and those of Bakst and Cecil, Morisson or Jaskowski, irrespective of fowl breed and examiners experience. Blom stain caused sperm head swelling and showed a drastic reduction in the proportion of live spermatozoa with normal morphology. The staining method had a greater influence on sperm morphology evaluation than the experience of the examiners. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：583 / 588

页数：6

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