A New Multilocus Sequence Typing Scheme and Its Application for the Characterization of Photobacterium damselae subsp damselae Associated with Mortalityin Cetaceans

被引：9

作者：

Alba, Patricia ^{[1
]}

Caprioli, Andrea ^{[1
]}

Cocumelli, Cristiano ^{[2
]}

Ianzano, Angela ^{[1
]}

Donati, Valentina ^{[1
]}

Scholl, Francesco ^{[2
]}

Sorbara, Luigi ^{[1
]}

Terracciano, Giuliana ^{[3
]}

Fichi, Gianluca ^{[3
]}

Di Nocera, Fabio ^{[4
]}

Franco, Alessia ^{[1
]}

Battisti, Antonio ^{[1
]}

机构：

[1] Ist Zooprofilatt Sperimentale Lazio & Toscana, Sede Cent, Gen Diagnost Dept, Rome, Italy

[2] Ist Zooprofilatt Sperimentale Lazio & Toscana, Sede Cent, Dept Pathol, Rome, Italy

[3] Ist Zooprofilatt Sperimentale Lazio & Toscana, Sez Pisa, Pisa, Italy

[4] Ist Zooprofilatt Sperimentale Mezzogiorno, Dept Anim Hlth, Portrci, Italy

来源：

FRONTIERS IN MICROBIOLOGY | 2016年 / 7卷

关键词：

Photobacterium damselae; MLST; genotyping; phylogeny; cetaceans; infection; zoonoses; DNA; EVOLUTION; VIBRIO; HEMOLYSINS; RESISTANCE; VIRULENCE; PLASMIDS; PATHOGENICITY; SEDIMENT; BACTERIA;

D O I：

10.3389/fmicb.2016.01656

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Photobacterium damselae subsp. damselae (PDD) is a known pathogen of fish, humans and marine mammals. In this study, a Multilocus Sequence Typing (MLST) scheme based on six housekeeping genes (glp, gyrB, metG, pnt, pyrC, and toxR) was developed to better understand the PDD population structure and used to type 73 PDD isolates from cetaceans, mainly striped dolphins (Stenella coeruleoalba) involved in mortality episodes, and from a few marine chelonians. Five reference ATCC strains were also included in the study. Typing allowed the discrimination of groups of PDD strains isolated from different host species, at different times and from different geographic areas, suggesting that a clonal PDD group may have spread in the Tyrrhenian sea at the time of an Unusual Mortality Event (UME) among cetaceans, mainly striped dolphins, occurred in early 2013 along the Italian western coasts.

引用

页数：10

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