Electrocatalytic evaluation of a horseradish peroxidase biosensor based on a novel Bi-Ag bimetallic nanocomposite

被引：6
作者：
van der Horst, Charlton [1 ,2 ]
Silwana, Bongiwe [1 ,2 ]
Iwuoha, Emmanuel [1 ]
Somerset, Vernon [2 ]
机构：
[1] Univ Western Cape, Dept Chem, SensorLab, ZA-7535 Bellville, South Africa
[2] CSIR, NRE, ZA-7600 Stellenbosch, South Africa
来源：
BIOSENSORS 2016 | 2017年 / 27卷
基金：
新加坡国家研究基金会;
关键词：
Differential pulse voltammetry; Horseradish peroxidase; Hydrogen peroxide; Bismuth-silver nanoparticles; Cyclic voltammetry; HYDROGEN-PEROXIDE; POLYMER; WATER;
D O I：
10.1016/j.protcy.2017.04.077
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
The present work illustrates the construction and application of bismuth-silver bimetallic nanoparticles (Bi-AgNPs) in biosensor construction for the detection of peroxide (H2O2). The biosensor was constructed by drop-casting of horseradish peroxidase onto the Bi-AgNPs/GCE sensor. Impedance analysis revealed that the R-ct value for the bare GCE obtained was 2.548 x 10(3) Omega and increased to 5.768 x 10(3) Omega for the HRP/Bi-AgNPs/GCE biosensor. This increased value of R-ct indicated hindrance to the electron transfer, providing evidence for the immobilization of insulating HRP on the Bi-AgNPs/GCE surface. The HRP/Bi-AgNPs/GCE biosensor was successfully applied for sensitive detection of H2O2 by differential pulse voltammetry. Well defined peaks were observed with a low linearity range from 0.02 x 10(-6) to 1.0 x 10(-6) M (R-2 = 0.991), with a limit of detection of 0.06 mu M. The results obtained for the HRP/Bi-AgNPs/GCE biosensor compared well within the range of similar biosensors reported.
引用
收藏
页码：179 / 182
页数：4
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