Electrocatalytic evaluation of a horseradish peroxidase biosensor based on a novel Bi-Ag bimetallic nanocomposite

被引：6

作者：

van der Horst, Charlton ^{[1
,2
]}

Silwana, Bongiwe ^{[1
,2
]}

Iwuoha, Emmanuel ^{[1
]}

Somerset, Vernon ^{[2
]}

机构：

[1] Univ Western Cape, Dept Chem, SensorLab, ZA-7535 Bellville, South Africa

[2] CSIR, NRE, ZA-7600 Stellenbosch, South Africa

来源：

BIOSENSORS 2016 | 2017年 / 27卷

基金：

新加坡国家研究基金会;

关键词：

Differential pulse voltammetry; Horseradish peroxidase; Hydrogen peroxide; Bismuth-silver nanoparticles; Cyclic voltammetry; HYDROGEN-PEROXIDE; POLYMER; WATER;

D O I：

10.1016/j.protcy.2017.04.077

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The present work illustrates the construction and application of bismuth-silver bimetallic nanoparticles (Bi-AgNPs) in biosensor construction for the detection of peroxide (H2O2). The biosensor was constructed by drop-casting of horseradish peroxidase onto the Bi-AgNPs/GCE sensor. Impedance analysis revealed that the R-ct value for the bare GCE obtained was 2.548 x 10(3) Omega and increased to 5.768 x 10(3) Omega for the HRP/Bi-AgNPs/GCE biosensor. This increased value of R-ct indicated hindrance to the electron transfer, providing evidence for the immobilization of insulating HRP on the Bi-AgNPs/GCE surface. The HRP/Bi-AgNPs/GCE biosensor was successfully applied for sensitive detection of H2O2 by differential pulse voltammetry. Well defined peaks were observed with a low linearity range from 0.02 x 10(-6) to 1.0 x 10(-6) M (R-2 = 0.991), with a limit of detection of 0.06 mu M. The results obtained for the HRP/Bi-AgNPs/GCE biosensor compared well within the range of similar biosensors reported.

引用

页码：179 / 182

页数：4

共 22 条

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