Antarcticimicrobium sediminis gen. nov., sp. nov., isolated from Antarctic intertidal sediment, transfer of Ruegeria lutea to Antarcticimicrobium gen. nov. as Antarcticimicrobium luteum comb. nov.

A Gram-stain-negative, aerobic, non-flagellated and rod- or ovoid-shaped bacterium, designated as strain S4J41(T). was isolated from Antarctic intertidal sediment. The isolate grew at 0-37 degrees C and with 0.5-10% (w/v) NaCl. It reduced nitrate to nitrite and hydrolysed Tween 80 and gelatin. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain S4J41(T) constituted a distinct phylogenetic line within the family Rhodobacteraceae and was closely related with some species in the genera Ruegeria, Phaeobacter, Pseudopuniceibacterium. Sulfitobacter. Puniceibacterium and Poseidonocella with 98.6-95.7% 16S rRNA gene sequence similarities. The major cellular fatty acids were C-16:0, summed feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c) and C-1(8:)0 and the major polar lipids were phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, phosphatidylethanolamine and one unidentified aminolipid. The sole respiratory quinone was 0-10. The genomic DNA G+C content of strain S4J41(T) was 60.3 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic data obtained in this study, strain S4J41(T) is considered to represent a novel species in a new genus within the family Rhodobacteraceae, for which the name Antarcticimicrobium sediminis gen. nov., sp. nov. is proposed. The type strain is S4J41(T) (=MCCC 1K03508(T)=KCTC 62793(T)). Moreover, the transfer of Ruegeria lutea Kim et al. 2019 to Antarcticimicrobium gen. nov. as Antarcticimicrobium luteum comb. nov. (type strain 318-1(T)=JCM 30927(T)=KCTC 72105(T)) is also proposed.