Occurrence and Genetic Diversity of Grapevine berry inner necrosis virus from Grapevines in China

被引:16
作者
Fan, X. D. [1 ,2 ]
Zhang, Z. P. [1 ]
Ren, F. [1 ]
Hu, G. J. [1 ]
Zhou, J. [1 ]
Li, Z. N. [1 ]
Wang, G. [2 ]
Dong, Yafeng [1 ]
机构
[1] Chinese Acad Sci, Res Inst Pomol, Natl Ctr Eliminating Viruses Deciduous Fruit Tree, Liaoning 125100, Xingcheng, Peoples R China
[2] Huazhong Agr Univ, Natl Key Lab Agromicrobiol, Coll Plant Sci & Technol, Wuhan 430070, Hubei, Peoples R China
关键词
1ST REPORT; PRIMERS;
D O I
10.1094/PDIS-05-16-0694-RE
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
To investigate the prevalence and genetic diversity of Grapevine berry inner necrosis virus (GINV) in China, 195 grapevine samples from 15 Chinese provinces and regions were tested using reverse-transcription polymerase chain reaction. The samples included symptomatic and asymptomatic cultivars, with 35.9% (70 of 195) of samples testing positive for GINV. Seventeen samples had obvious ring spot symptoms, and 94.1% (16 of 17) tested positive for GINV, suggesting that GINV may be highly associated with the ring spot symptom. The genetic diversity of GINV isolates was analyzed based on the partial nucleotide and amino acid sequences of the coat protein (CP) and movement protein (MP) genes. Phylogenetic analyses of the MP and CP gene sequences divided the GINV isolates into three groups. The majority of the Chinese.isolates were in groups 1 and 2, and only one Chinese isolate, along with a previously reported Japanese isolate, was in group 3. This is the first report on the genetic diversity of GINV isolates and their prevalence and distribution in China.
引用
收藏
页码:144 / 149
页数:6
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