Ginsenoside Rd inhibits the expressions of iNOS and COX-2 by suppressing NF-κB in LPS-stimulated RAW264.7 cells and mouse liver

被引：132

作者：

Kim, Dae Hyun ^{[1
]}

Chung, Jae Heun ^{[1
]}

Yoon, Ji Sung ^{[1
]}

Ha, Young Mi ^{[1
]}

Bae, Sungjin ^{[1
]}

Lee, Eun Kyeong ^{[2
]}

Jung, Kyung Jin ^{[3
]}

Kim, Min Sun ^{[4
]}

Kim, You Jung ^{[5
]}

Kim, Mi Kyung ^{[6
]}

Chung, Hae Young ^{[1
,6
]}

机构：

[1] Pusan Natl Univ, Coll Pharm, Mol Inflammat Res Ctr Aging Intervent MRCA, Pusan 609735, South Korea

[2] Dongnam Inst Radiol & Med Sci, Res Ctr, Pusan 619953, South Korea

[3] Korea Inst Toxicol, Inhalat Toxicol Ctr, Jeongeup 580185, South Korea

[4] Sunchon Natl Univ, Dept Pharm, Coll Pharm, Sunchon 540742, South Korea

[5] Busan Womens Coll, Dept Dent Hyg, Pusan 614734, South Korea

[6] Pusan Natl Univ, Longev Life Sci & Technol Inst, Pusan 609735, South Korea

来源：

JOURNAL OF GINSENG RESEARCH | 2013年 / 37卷 / 01期

关键词：

Panax ginseng; Ginsenoside Rd; Inducible nitric oxide synthase; Cyclooxygenase-2; Prostaglandin E-2;

D O I：

10.5142/jgr.2013.37.54

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Ginsenoside Rd is a primary constituent of the ginseng rhizome and has been shown to participate in the regulation of diabetes and in tumor formation. Reports also show that ginsenoside Rd exerts anti-oxidative effects by activating anti-oxidant enzymes. Treatment with ginsenoside Rd decreased nitric oxide and prostaglandin E-2 (PGE(2)) in lipopolysaccharides (LPS)-challenged RAW264.7 cells and in ICR mouse livers (5 mg/kg LPS; LPS + ginsenoside Rd [2, 10, and 50 mg/kg]). Furthermore, these decreases were associated with the down-regulations of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 and of nuclear factor (NF)-kappa B activity in vitro and in vivo. Our results indicate that ginsenoside Rd treatment decreases; 1) nitric oxide production (40% inhibition); 2) PGE(2) synthesis (69% to 93% inhibition); 3) NF-kappa B activity; and 4) the NF-kappa B-regulated expressions of iNOS and COX-2. Taken together, our results suggest that the anti-inflammatory effects of ginsenoside Rd are due to the down-regulation of NF-kappa B and the consequent expressional suppressions of iNOS and COX-2.

引用

页码：54 / 63

页数：10

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