Purification and biochemical characterization of a cold-active lipase from Antarctic sea ice bacteria Pseudoalteromonas sp NJ 70

被引：33

作者：

Wang, Quanfu ^{[1
,2
]}

Hou, Yanhua ^{[1
]}

Ding, Yu ^{[3
]}

Yan, Peisheng ^{[1
]}

机构：

[1] Harbin Inst Technol, Sch Marine & Technol, Weihai 264209, Peoples R China

[2] Harbin Inst Technol, Sch Chem Engn, Harbin 150001, Peoples R China

[3] Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524025, Guangdong, Peoples R China

来源：

MOLECULAR BIOLOGY REPORTS | 2012年 / 39卷 / 09期

基金：

中国博士后科学基金; 中国国家自然科学基金;

关键词：

Purification; Antarctic; Pseudoalteromonas sp; Sea ice; Cold-active lipase; ADAPTED LIPASE; GENE CLONING; PSYCHROTROPHIC BACTERIUM; PSYCHROPHILIC BACTERIUM; ESCHERICHIA-COLI; EXPRESSION; ENZYME;

D O I：

10.1007/s11033-012-1796-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An extracellular cold-active lipase from Antarctic sea ice bacteria Pseudoalteromonas sp. NJ 70 was purified and characterized. The overall purification based on lipase activity was 27.5-fold with a yield of 25.4 %. The purified lipase showed as a single band on SDS-PAGE with an apparent molecular weight of 37 kDa. The optimum temperature and pH were 35 A degrees C and 7.0, respectively. The lipase activity was enhanced by Ca2+ and Mg2+, while was partially inhibited by other metals such as Cu2+, Zn2+, Ba2+, Pb2+, Fe2+ and Mn2+. The lipase had high tolerance to a wide range of NaCl concentrations (0-2 M NaCl). It exhibited high levels of activity in the presence of DTT, Thiourea, H2O2 as well as in the presence of various detergents such as Span 60, Tween-80, Triton X-100. In addition, the lipase showed a preference for long-chain p-nitrophenyl esters (C-12-C-18). These results indicated that this lipase could be a novel cold-active lipase.

引用

页码：9233 / 9238

页数：6

共 27 条

[1] A cold-adapted esterase of a novel marine isolate, Pseudoalteromonas arctica: gene cloning, enzyme purification and characterization [J].

Al Khudary, Rami ;

Venkatachalam, Ramprasath ;

Katzer, Moritz ;

Elleuche, Skander ;

Antranikian, Garabed .

EXTREMOPHILES, 2010, 14 (03) :273-285

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3] Biotechnological uses of enzymes from psychrophiles [J].

Cavicchioli, R. ;

Charlton, T. ;

Ertan, H. ;

Omar, S. Mohd ;

Siddiqui, K. S. ;

Williams, T. J. .

MICROBIAL BIOTECHNOLOGY, 2011, 4 (04) :449-460

[4] Gene cloning, expression and characterization of a cold-adapted lipase from a psychrophilic deep-sea bacterium Psychrobacter sp C18 [J].

Chen, Ruipeng ;

Guo, Lizhong ;

Dang, Hongyue .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2011, 27 (02) :431-441

[5]

Choo DW, 1998, APPL ENVIRON MICROB, V64, P486

[6] A cold-adapted esterase from psychrotrophic Pseudoalteromas sp strain 643A [J].

Cieslinski, Hubert ;

Bialkowska, Aneta M. ;

Dlugolecka, Anna ;

Daroch, Maurycy ;

Tkaczuk, Karolina L. ;

Kalinowska, Halina ;

Kur, Jozef ;

Turkiewicz, Marianna .

ARCHIVES OF MICROBIOLOGY, 2007, 188 (01) :27-36

[7] The cold-active Lip1 lipase from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 is a member of a new bacterial lipolytic enzyme family [J].

de Pascale, Donatella ;

Cusano, Angela M. ;

Autore, Flavia ;

Parrilli, Ermenegilda ;

di Prisco, Guido ;

Marino, Gennaro ;

Tutino, M. Luisa .

EXTREMOPHILES, 2008, 12 (03) :311-323

[8] CLONING AND EXPRESSION IN ESCHERICHIA-COLI OF 3 LIPASE-ENCODING GENES FROM THE PSYCHROTROPHIC ANTARCTIC STRAIN MORAXELLA TA144 [J].

FELLER, G ;

THIRY, M ;

ARPIGNY, JL ;

GERDAY, C .

GENE, 1991, 102 (01) :111-115

[9]

Gunasekaran Vijay, 2006, Indian Journal of Experimental Biology, V44, P485

[10] Lipases for biotechnology [J].

Jaeger, KE ;

Eggert, T .

CURRENT OPINION IN BIOTECHNOLOGY, 2002, 13 (04) :390-397

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