Separation of myoglobin and hemoglobin in isolated perfused pig heart

被引:3
作者
Rauh, R [1 ]
Hiller, M [1 ]
Trinks, T [1 ]
Kessler, M [1 ]
机构
[1] Univ Erlangen Nurnberg, Inst Physiol & Cardiol, D-91054 Erlangen, Germany
来源
DIAGNOSTIC OPTICAL SPECTROSCOPY IN BIOMEDICINE | 2001年 / 4432卷
关键词
hemoglobin; 3D-imaging; pig heart; isolated perfusion; physiology;
D O I
10.1117/12.447147
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
For the quantitative determination of hemoglobin concentration in heart muscle it is important to distinguish between myoglobin and hemoglobin, two dyes with very similar optical absorption properties. With an isolated perfused pig heart model and EMPHO II SSK we measured tissue spectra in the visible range before and after adding erythrocytes to the perfusate. By calculating light intensity differences we were able to show spatial hemoglobin distribution in heart muscle.
引用
收藏
页码:263 / 267
页数:5
相关论文
共 6 条
[1]   THE ERLANGEN MICRO-LIGHTGUIDE SPECTROPHOTOMETER EMPHO-I [J].
FRANK, KH ;
KESSLER, M ;
APPELBAUM, K ;
DUMMLER, W .
PHYSICS IN MEDICINE AND BIOLOGY, 1989, 34 (12) :1883-1900
[2]   Electronic light microscopy combined with spectrophotometry allows real-time analysis of structures at the subcellular level [J].
Funk, RHW ;
Hoper, J ;
Dramm, P ;
Hofer, A .
PHYSIOLOGICAL MEASUREMENT, 1998, 19 (02) :225-233
[3]  
KESSLER M, 2001, P SPIE, V4241
[4]   Optical investigation of functional structures in isolated perfused pig heart [J].
Rauh, R ;
Boehnert, M ;
Mahlke, C ;
Kessler, M .
CONTROLLING TISSUE OPTICAL PROPERTIES: APPLICATIONS IN CLINICAL STUDY, 2000, 4162 :205-211
[5]  
RAUH R, 2001, P SPIE, V4241
[6]   CARDIOPLEGIA ON THE CONTRACTILE APPARATUS LEVEL - EVALUATION OF A NEW CONCEPT FOR MYOCARDIAL PRESERVATION IN PERFUSED PIG HEARTS [J].
VAHL, CF ;
BONZ, A ;
HAGL, C ;
TIMEK, T ;
HEROLD, U ;
FUCHS, H ;
KOCHSIEK, N ;
HAGL, S .
THORACIC AND CARDIOVASCULAR SURGEON, 1995, 43 (04) :185-193