Mechanical stimulation of single cells by reversible host-guest interactions in 3D microscaffolds

被引：74

作者：

Hippler, Marc ^{[1
,2
]}

Weissenbruch, Kai ^{[2
,3
]}

Richler, Kai ^{[2
]}

Lemma, Enrico D. ^{[2
]}

Nakahata, Masaki ^{[4
]}

Richter, Benjamin ^{[2
]}

Barner-Kowollik, Christopher ^{[5
,6
,7
]}

Takashima, Yoshinori ^{[8
]}

Harada, Akira ^{[8
]}

Blasco, Eva ^{[7
,9
]}

Wegener, Martin ^{[1
,9
]}

Tanaka, Motomu ^{[10
,11
]}

Bastmeyer, Martin ^{[2
,3
]}

机构：

[1] Karlsruhe Inst Technol KIT, Inst Appl Phys, D-76128 Karlsruhe, Germany

[2] Karlsruhe Inst Technol KIT, Zool Inst, D-76128 Karlsruhe, Germany

[3] Karlsruhe Inst Technol KIT, Inst Funct Interfaces, D-76128 Karlsruhe, Germany

[4] Osaka Univ, Grad Sch Engn Sci, Dept Mat Engn Sci, Toyonaka, Osaka 5608531, Japan

[5] Queensland Univ Technol QUT, Ctr Mat Sci, 2 George St, Brisbane, Qld 4000, Australia

[6] Queensland Univ Technol QUT, Sch Chem & Phys, 2 George St, Brisbane, Qld 4000, Australia

[7] Karlsruhe Inst Technol KIT, Inst Nanotechnol, D-76128 Karlsruhe, Germany

[8] Osaka Univ, Grad Sch Sci, Dept Macromol Sci, 1-1 Machikaneyama Cho, Toyonaka, Osaka 5600043, Japan

[9] Karlsruhe Inst Technol KIT, Inst Chem Technol & Polymer Chem, D-76128 Karlsruhe, Germany

[10] Heidelberg Univ, Inst Phys Chem, Neuenheimer Feld 253, D-69120 Heidelberg, Germany

[11] Kyoto Univ, Ctr Integrat Med & Phys, Inst Adv Study, Kyoto 6068501, Japan

来源：

SCIENCE ADVANCES | 2020年 / 6卷 / 39期

基金：

澳大利亚研究理事会;

关键词：

TENSIONAL HOMEOSTASIS; CELLULAR ADAPTATION; FIBROBLASTS; STRESS; RHO; RESPONSES; KINASE; FORCE;

D O I：

10.1126/sciadv.abc2648

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Many essential cellular processes are regulated by mechanical properties of their microenvironment. Here, we introduce stimuli-responsive composite scaffolds fabricated by three-dimensional (3D) laser lithography to simultaneously stretch large numbers of single cells in tailored 3D microenvironments. The key material is a stimuli-responsive photoresist containing cross-links formed by noncovalent, directional interactions between.-cyclodextrin (host) and adamantane (guest). This allows reversible actuation under physiological conditions by application of soluble competitive guests. Cells adhering in these scaffolds build up initial traction forces of similar to 80 nN. After application of an equibiaxial stretch of up to 25%, cells remodel their actin cytoskeleton, double their traction forces, and equilibrate at a new dynamic set point within 30 min. When the stretch is released, traction forces gradually decrease until the initial set point is retrieved. Pharmacological inhibition or knockout of nonmuscle myosin 2A prevents these adjustments, suggesting that cellular tensional homeostasis strongly depends on functional myosin motors.

引用

页数：13

共 48 条

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