Spectral discrimination of live prostate and bladder cancer cell lines using Raman optical tweezers

被引：63

作者：

Harvey, Tim J. ^{[1
]}

Faria, Elsa Correia ^{[1
]}

Henderson, Alex ^{[1
]}

Gazi, Ehsan ^{[2
]}

Ward, Andrew D. ^{[3
]}

Clarke, Noel W. ^{[2
]}

Brown, Michael D. ^{[2
]}

Snook, Richard D. ^{[1
]}

Gardner, Peter ^{[1
]}

机构：

[1] Univ Manchester, Sch Chem Engn & Analyt Sci, Manchester Interdisciplinary Bioctr, Manchester M1 7DN, Lancs, England

[2] Univ Manchester, Christie Hosp NHS Trust, Paterson Inst Canc Res, Genito Urinary Canc Res Grp, Manchester M20 4BX, Lancs, England

[3] Cent Laser Facil, Rutherford Appleton Lab, Sci & Technol Facil Council, Didcot OX11 OPX, Oxon, England

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2008年 / 13卷 / 06期

基金：

英国医学研究理事会;

关键词：

Raman optical tweezers; vibrational spectroscopy; prostate cancer; cell lines; principal component analysis; chemometrics;

D O I：

10.1117/1.2999609

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An investigation into the use of Raman optical tweezers to study urological cell lines is reported, with the ultimate aim of determining the presence of malignant CaP cells in urine and peripheral fluids. To this end, we trapped and analyzed live CaP cells (PC-3) and bladder cells (MGH-U1), because both prostate and bladder cells are likely to be present in urine. The laser excitation wavelength of 514.5 nm was used, with Raman light collected both in back- and forward-scattering geometric configurations. For the backscattering configuration the same laser was used for trapping and excitation, while for forward scattering a 1064 nm laser provided the trapping beam. Analysis of cell-diameter distributions for cells analyzed suggested normal distribution of cell sizes, indicating an unbiased cell-selection criterion. Principal components analysis afforded discrimination of MGH-U1 and PC-3 spectra collected in either configuration, demonstrating that it is possible to trap, analyze, and differentiate PC-3 from MGH-U1 cells using a 514.5 nm laser. By loading plot analysis, possible biomolecules responsible for discrimination in both configurations were determined. Finally, the effect of cell size on discrimination was investigated, with results indicating that separation is based predominantly on cell type rather than cell size. (C) 2008 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.2999609]

引用

页数：12

共 62 条

[1] Raman spectra of biological samples: A study of preprocessing methods [J].